Protocol for design, construction, and selection of genome phage (gPhage) display libraries
Summary: This protocol describes the genomic phage (gPhage) display platform, a large-scale antigen and epitope mapping technique. We constructed a gPhage display peptide library of a eukaryotic organism, Trypanosoma cruzi (causative agent of Chagas disease), to map the antibody response landscape a...
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2021
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oai:doaj.org-article:d9424f25152a4e7cb7d4a8716980ff322021-11-10T04:41:29ZProtocol for design, construction, and selection of genome phage (gPhage) display libraries2666-166710.1016/j.xpro.2021.100936https://doaj.org/article/d9424f25152a4e7cb7d4a8716980ff322021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2666166721006420https://doaj.org/toc/2666-1667Summary: This protocol describes the genomic phage (gPhage) display platform, a large-scale antigen and epitope mapping technique. We constructed a gPhage display peptide library of a eukaryotic organism, Trypanosoma cruzi (causative agent of Chagas disease), to map the antibody response landscape against the parasite. Here, we used an organism with a relatively large but intronless genome, although future applications could include other prevalent or (re)emerging infectious organisms carrying genomes with a limited number of introns.For complete details on the use and execution of this protocol, please refer to Teixeira et al. (2021).Luis Antonio Rodriguez CarneroAndré Azevedo Reis TeixeiraFenny Hui Fen TangAndréia KuramotoMaria Júlia Manso AlvesWalter ColliJoão Carlos SetubalEdécio Cunha-NetoRenata PasqualiniWadih ArapRicardo José GiordanoElsevierarticleSequence analysisGenomicsSequencingHigh Throughput ScreeningImmunologyMicrobiologyScience (General)Q1-390ENSTAR Protocols, Vol 2, Iss 4, Pp 100936- (2021) |
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DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
Sequence analysis Genomics Sequencing High Throughput Screening Immunology Microbiology Science (General) Q1-390 |
| spellingShingle |
Sequence analysis Genomics Sequencing High Throughput Screening Immunology Microbiology Science (General) Q1-390 Luis Antonio Rodriguez Carnero André Azevedo Reis Teixeira Fenny Hui Fen Tang Andréia Kuramoto Maria Júlia Manso Alves Walter Colli João Carlos Setubal Edécio Cunha-Neto Renata Pasqualini Wadih Arap Ricardo José Giordano Protocol for design, construction, and selection of genome phage (gPhage) display libraries |
| description |
Summary: This protocol describes the genomic phage (gPhage) display platform, a large-scale antigen and epitope mapping technique. We constructed a gPhage display peptide library of a eukaryotic organism, Trypanosoma cruzi (causative agent of Chagas disease), to map the antibody response landscape against the parasite. Here, we used an organism with a relatively large but intronless genome, although future applications could include other prevalent or (re)emerging infectious organisms carrying genomes with a limited number of introns.For complete details on the use and execution of this protocol, please refer to Teixeira et al. (2021). |
| format |
article |
| author |
Luis Antonio Rodriguez Carnero André Azevedo Reis Teixeira Fenny Hui Fen Tang Andréia Kuramoto Maria Júlia Manso Alves Walter Colli João Carlos Setubal Edécio Cunha-Neto Renata Pasqualini Wadih Arap Ricardo José Giordano |
| author_facet |
Luis Antonio Rodriguez Carnero André Azevedo Reis Teixeira Fenny Hui Fen Tang Andréia Kuramoto Maria Júlia Manso Alves Walter Colli João Carlos Setubal Edécio Cunha-Neto Renata Pasqualini Wadih Arap Ricardo José Giordano |
| author_sort |
Luis Antonio Rodriguez Carnero |
| title |
Protocol for design, construction, and selection of genome phage (gPhage) display libraries |
| title_short |
Protocol for design, construction, and selection of genome phage (gPhage) display libraries |
| title_full |
Protocol for design, construction, and selection of genome phage (gPhage) display libraries |
| title_fullStr |
Protocol for design, construction, and selection of genome phage (gPhage) display libraries |
| title_full_unstemmed |
Protocol for design, construction, and selection of genome phage (gPhage) display libraries |
| title_sort |
protocol for design, construction, and selection of genome phage (gphage) display libraries |
| publisher |
Elsevier |
| publishDate |
2021 |
| url |
https://doaj.org/article/d9424f25152a4e7cb7d4a8716980ff32 |
| work_keys_str_mv |
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