Off-the-shelf proximity biotinylation for interaction proteomics

Proximity biotinylation is a powerful tool to profile interactomes, but it requires genetic engineering of the target protein. Here, the authors develop a proximity biotinylation enzyme that can be directed to the target using antibodies, enabling interactome profiling of endogenous proteins or PTMs...

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Auteurs principaux:	Irene Santos-Barriopedro, Guido van Mierlo, Michiel Vermeulen
Format:	article
Langue:	EN
Publié:	Nature Portfolio 2021
Sujets:	Science Q
Accès en ligne:	https://doaj.org/article/daee1039e77c42bc92b128ce138c9f8d
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spelling	oai:doaj.org-article:daee1039e77c42bc92b128ce138c9f8d2021-12-02T16:45:42ZOff-the-shelf proximity biotinylation for interaction proteomics10.1038/s41467-021-25338-42041-1723https://doaj.org/article/daee1039e77c42bc92b128ce138c9f8d2021-08-01T00:00:00Zhttps://doi.org/10.1038/s41467-021-25338-4https://doaj.org/toc/2041-1723Proximity biotinylation is a powerful tool to profile interactomes, but it requires genetic engineering of the target protein. Here, the authors develop a proximity biotinylation enzyme that can be directed to the target using antibodies, enabling interactome profiling of endogenous proteins or PTMs.Irene Santos-BarriopedroGuido van MierloMichiel VermeulenNature PortfolioarticleScienceQENNature Communications, Vol 12, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Irene Santos-Barriopedro Guido van Mierlo Michiel Vermeulen Off-the-shelf proximity biotinylation for interaction proteomics
description	Proximity biotinylation is a powerful tool to profile interactomes, but it requires genetic engineering of the target protein. Here, the authors develop a proximity biotinylation enzyme that can be directed to the target using antibodies, enabling interactome profiling of endogenous proteins or PTMs.
format	article
author	Irene Santos-Barriopedro Guido van Mierlo Michiel Vermeulen
author_facet	Irene Santos-Barriopedro Guido van Mierlo Michiel Vermeulen
author_sort	Irene Santos-Barriopedro
title	Off-the-shelf proximity biotinylation for interaction proteomics
title_short	Off-the-shelf proximity biotinylation for interaction proteomics
title_full	Off-the-shelf proximity biotinylation for interaction proteomics
title_fullStr	Off-the-shelf proximity biotinylation for interaction proteomics
title_full_unstemmed	Off-the-shelf proximity biotinylation for interaction proteomics
title_sort	off-the-shelf proximity biotinylation for interaction proteomics
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/daee1039e77c42bc92b128ce138c9f8d
work_keys_str_mv	AT irenesantosbarriopedro offtheshelfproximitybiotinylationforinteractionproteomics AT guidovanmierlo offtheshelfproximitybiotinylationforinteractionproteomics AT michielvermeulen offtheshelfproximitybiotinylationforinteractionproteomics
_version_	1718383485249388544

Off-the-shelf proximity biotinylation for interaction proteomics

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