Off-the-shelf proximity biotinylation for interaction proteomics
Proximity biotinylation is a powerful tool to profile interactomes, but it requires genetic engineering of the target protein. Here, the authors develop a proximity biotinylation enzyme that can be directed to the target using antibodies, enabling interactome profiling of endogenous proteins or PTMs...
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Main Authors: | Irene Santos-Barriopedro, Guido van Mierlo, Michiel Vermeulen |
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Format: | article |
Language: | EN |
Published: |
Nature Portfolio
2021
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Subjects: | |
Online Access: | https://doaj.org/article/daee1039e77c42bc92b128ce138c9f8d |
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