Flavin mononucleotide-based fluorescent proteins function in mammalian cells without oxygen requirement.

Usage of the enhanced green fluorescent protein (eGFP) in living mammalian cells is limited to aerobic conditions due to requirement of oxygen during chromophore formation. Since many diseases or disease models are associated with acute or chronic hypoxia, eGFP-labeling of structures of interest in...

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Autores principales: Janine Walter, Sascha Hausmann, Thomas Drepper, Michael Puls, Thorsten Eggert, Marcel Dihné
Formato: article
Lenguaje:EN
Publicado: Public Library of Science (PLoS) 2012
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Acceso en línea:https://doaj.org/article/db363103e0584e55b89d298090ce9e00
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