Dissociation of intact adult mouse cortical projection neurons for single-cell RNA-seq

Summary: This protocol provides an improved pipeline for dissociating intact projection neurons from adult mouse cortex for applications including droplet and plate-based single-cell RNA sequencing, qPCR, immunocytochemistry, and long-term in vitro cell culture. This protocol provides a robust and r...

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Autores principales: Noa Golan, William B. Cafferty
Formato: article
Lenguaje:EN
Publicado: Elsevier 2021
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Acceso en línea:https://doaj.org/article/dc0fab82d2094529a55604614fe134ca
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