The Efficiency of Selected Extenders against Bacterial Contamination of Boar Semen in a Swine Breeding Facility in Western Slovakia

Bacteriospermia has become a serious factor affecting sperm quality in swine breeding, this is why antibiotics (ATBs) are a critical component of semen extenders. Due to ever-increasing antimicrobial resistance, the aim of this study was to assess the efficiency of selected commercially available se...

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Autores principales:	Eva Tvrdá, Ondřej Bučko, Kristína Rojková, Michal Ďuračka, Simona Kunová, Ján Kováč, Filip Benko, Miroslava Kačániová
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	bacteriospermia boars semen extender antibiotics MALDI-TOF bacterial resistance Veterinary medicine SF600-1100 Zoology QL1-991
Acceso en línea:	https://doaj.org/article/dcdc115a76c84b58b8daee025bf071e0
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spelling	oai:doaj.org-article:dcdc115a76c84b58b8daee025bf071e02021-11-25T16:21:29ZThe Efficiency of Selected Extenders against Bacterial Contamination of Boar Semen in a Swine Breeding Facility in Western Slovakia10.3390/ani111133202076-2615https://doaj.org/article/dcdc115a76c84b58b8daee025bf071e02021-11-01T00:00:00Zhttps://www.mdpi.com/2076-2615/11/11/3320https://doaj.org/toc/2076-2615Bacteriospermia has become a serious factor affecting sperm quality in swine breeding, this is why antibiotics (ATBs) are a critical component of semen extenders. Due to ever-increasing antimicrobial resistance, the aim of this study was to assess the efficiency of selected commercially available semen extenders to prevent a possible bacterial contamination of boar ejaculates. Three Androstar Plus extenders containing different combinations of antibiotics were used to process ejaculates from 30 healthy Duroc breeding boars. Androstar Plus without antibiotics was used as a control. The extended samples were stored at 17 °C for 72 h. Sperm motility, viability, mitochondrial activity, DNA integrity and oxidative profile of each extended sample were assessed following 24 h, 48 h and 72 h. Furthermore, selective media were used to quantify the bacterial load and specific bacterial species were identified with matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry. The results indicate that semen extenders enriched with ATBs ensured a significantly higher preservation of the sperm quality in comparison to the ATB-free control. The total bacterial count was significantly decreased in the extenders supplemented with ATBs (<i>p </i>< 0.001), however gentamycin alone was not effective enough against Gram-positive bacteria, while a few colonies of <i>Enterococcus hirae</i>, <i>Bacillus subtilis</i> and <i>Corynebacterium</i> spp. were present in the samples extended in the presence of a triple combination of ATBs. In conclusion, we may suggest that semen extenders enriched in antibiotics were not able to fully eliminate the bacteria present in the studied samples. Furthermore, selection of suitable antibiotics for semen extension should be accompanied by adequate hygiene standards during the collection and handling of boar ejaculates.Eva TvrdáOndřej BučkoKristína RojkováMichal ĎuračkaSimona KunováJán KováčFilip BenkoMiroslava KačániováMDPI AGarticlebacteriospermiaboarssemen extenderantibioticsMALDI-TOFbacterial resistanceVeterinary medicineSF600-1100ZoologyQL1-991ENAnimals, Vol 11, Iss 3320, p 3320 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	bacteriospermia boars semen extender antibiotics MALDI-TOF bacterial resistance Veterinary medicine SF600-1100 Zoology QL1-991
spellingShingle	bacteriospermia boars semen extender antibiotics MALDI-TOF bacterial resistance Veterinary medicine SF600-1100 Zoology QL1-991 Eva Tvrdá Ondřej Bučko Kristína Rojková Michal Ďuračka Simona Kunová Ján Kováč Filip Benko Miroslava Kačániová The Efficiency of Selected Extenders against Bacterial Contamination of Boar Semen in a Swine Breeding Facility in Western Slovakia
description	Bacteriospermia has become a serious factor affecting sperm quality in swine breeding, this is why antibiotics (ATBs) are a critical component of semen extenders. Due to ever-increasing antimicrobial resistance, the aim of this study was to assess the efficiency of selected commercially available semen extenders to prevent a possible bacterial contamination of boar ejaculates. Three Androstar Plus extenders containing different combinations of antibiotics were used to process ejaculates from 30 healthy Duroc breeding boars. Androstar Plus without antibiotics was used as a control. The extended samples were stored at 17 °C for 72 h. Sperm motility, viability, mitochondrial activity, DNA integrity and oxidative profile of each extended sample were assessed following 24 h, 48 h and 72 h. Furthermore, selective media were used to quantify the bacterial load and specific bacterial species were identified with matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry. The results indicate that semen extenders enriched with ATBs ensured a significantly higher preservation of the sperm quality in comparison to the ATB-free control. The total bacterial count was significantly decreased in the extenders supplemented with ATBs (<i>p </i>< 0.001), however gentamycin alone was not effective enough against Gram-positive bacteria, while a few colonies of <i>Enterococcus hirae</i>, <i>Bacillus subtilis</i> and <i>Corynebacterium</i> spp. were present in the samples extended in the presence of a triple combination of ATBs. In conclusion, we may suggest that semen extenders enriched in antibiotics were not able to fully eliminate the bacteria present in the studied samples. Furthermore, selection of suitable antibiotics for semen extension should be accompanied by adequate hygiene standards during the collection and handling of boar ejaculates.
format	article
author	Eva Tvrdá Ondřej Bučko Kristína Rojková Michal Ďuračka Simona Kunová Ján Kováč Filip Benko Miroslava Kačániová
author_facet	Eva Tvrdá Ondřej Bučko Kristína Rojková Michal Ďuračka Simona Kunová Ján Kováč Filip Benko Miroslava Kačániová
author_sort	Eva Tvrdá
title	The Efficiency of Selected Extenders against Bacterial Contamination of Boar Semen in a Swine Breeding Facility in Western Slovakia
title_short	The Efficiency of Selected Extenders against Bacterial Contamination of Boar Semen in a Swine Breeding Facility in Western Slovakia
title_full	The Efficiency of Selected Extenders against Bacterial Contamination of Boar Semen in a Swine Breeding Facility in Western Slovakia
title_fullStr	The Efficiency of Selected Extenders against Bacterial Contamination of Boar Semen in a Swine Breeding Facility in Western Slovakia
title_full_unstemmed	The Efficiency of Selected Extenders against Bacterial Contamination of Boar Semen in a Swine Breeding Facility in Western Slovakia
title_sort	efficiency of selected extenders against bacterial contamination of boar semen in a swine breeding facility in western slovakia
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/dcdc115a76c84b58b8daee025bf071e0
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The Efficiency of Selected Extenders against Bacterial Contamination of Boar Semen in a Swine Breeding Facility in Western Slovakia

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