Par-1 regulates tissue growth by influencing hippo phosphorylation status and hippo-salvador association.
The evolutionarily conserved Hippo (Hpo) signaling pathway plays a pivotal role in organ size control by balancing cell proliferation and cell death. Here, we reported the identification of Par-1 as a regulator of the Hpo signaling pathway using a gain-of-function EP screen in Drosophila melanogaste...
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2013
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oai:doaj.org-article:dd46bc48791b4ce0879fd71e2a336dcc2021-11-18T05:37:54ZPar-1 regulates tissue growth by influencing hippo phosphorylation status and hippo-salvador association.1544-91731545-788510.1371/journal.pbio.1001620https://doaj.org/article/dd46bc48791b4ce0879fd71e2a336dcc2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23940457/pdf/?tool=EBIhttps://doaj.org/toc/1544-9173https://doaj.org/toc/1545-7885The evolutionarily conserved Hippo (Hpo) signaling pathway plays a pivotal role in organ size control by balancing cell proliferation and cell death. Here, we reported the identification of Par-1 as a regulator of the Hpo signaling pathway using a gain-of-function EP screen in Drosophila melanogaster. Overexpression of Par-1 elevated Yorkie activity, resulting in increased Hpo target gene expression and tissue overgrowth, while loss of Par-1 diminished Hpo target gene expression and reduced organ size. We demonstrated that par-1 functioned downstream of fat and expanded and upstream of hpo and salvador (sav). In addition, we also found that Par-1 physically interacted with Hpo and Sav and regulated the phosphorylation of Hpo at Ser30 to restrict its activity. Par-1 also inhibited the association of Hpo and Sav, resulting in Sav dephosphorylation and destabilization. Furthermore, we provided evidence that Par-1-induced Hpo regulation is conserved in mammalian cells. Taken together, our findings identified Par-1 as a novel component of the Hpo signaling network.Hong-Ling HuangShimin WangMeng-Xin YinLiang DongChao WangWei WuYi LuMiao FengChuanyang DaiXiaocan GuoLi LiBin ZhaoZhaocai ZhouHongbin JiJin JiangYun ZhaoXin-Yuan LiuLei ZhangPublic Library of Science (PLoS)articleBiology (General)QH301-705.5ENPLoS Biology, Vol 11, Iss 8, p e1001620 (2013) |
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Biology (General) QH301-705.5 |
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Biology (General) QH301-705.5 Hong-Ling Huang Shimin Wang Meng-Xin Yin Liang Dong Chao Wang Wei Wu Yi Lu Miao Feng Chuanyang Dai Xiaocan Guo Li Li Bin Zhao Zhaocai Zhou Hongbin Ji Jin Jiang Yun Zhao Xin-Yuan Liu Lei Zhang Par-1 regulates tissue growth by influencing hippo phosphorylation status and hippo-salvador association. |
| description |
The evolutionarily conserved Hippo (Hpo) signaling pathway plays a pivotal role in organ size control by balancing cell proliferation and cell death. Here, we reported the identification of Par-1 as a regulator of the Hpo signaling pathway using a gain-of-function EP screen in Drosophila melanogaster. Overexpression of Par-1 elevated Yorkie activity, resulting in increased Hpo target gene expression and tissue overgrowth, while loss of Par-1 diminished Hpo target gene expression and reduced organ size. We demonstrated that par-1 functioned downstream of fat and expanded and upstream of hpo and salvador (sav). In addition, we also found that Par-1 physically interacted with Hpo and Sav and regulated the phosphorylation of Hpo at Ser30 to restrict its activity. Par-1 also inhibited the association of Hpo and Sav, resulting in Sav dephosphorylation and destabilization. Furthermore, we provided evidence that Par-1-induced Hpo regulation is conserved in mammalian cells. Taken together, our findings identified Par-1 as a novel component of the Hpo signaling network. |
| format |
article |
| author |
Hong-Ling Huang Shimin Wang Meng-Xin Yin Liang Dong Chao Wang Wei Wu Yi Lu Miao Feng Chuanyang Dai Xiaocan Guo Li Li Bin Zhao Zhaocai Zhou Hongbin Ji Jin Jiang Yun Zhao Xin-Yuan Liu Lei Zhang |
| author_facet |
Hong-Ling Huang Shimin Wang Meng-Xin Yin Liang Dong Chao Wang Wei Wu Yi Lu Miao Feng Chuanyang Dai Xiaocan Guo Li Li Bin Zhao Zhaocai Zhou Hongbin Ji Jin Jiang Yun Zhao Xin-Yuan Liu Lei Zhang |
| author_sort |
Hong-Ling Huang |
| title |
Par-1 regulates tissue growth by influencing hippo phosphorylation status and hippo-salvador association. |
| title_short |
Par-1 regulates tissue growth by influencing hippo phosphorylation status and hippo-salvador association. |
| title_full |
Par-1 regulates tissue growth by influencing hippo phosphorylation status and hippo-salvador association. |
| title_fullStr |
Par-1 regulates tissue growth by influencing hippo phosphorylation status and hippo-salvador association. |
| title_full_unstemmed |
Par-1 regulates tissue growth by influencing hippo phosphorylation status and hippo-salvador association. |
| title_sort |
par-1 regulates tissue growth by influencing hippo phosphorylation status and hippo-salvador association. |
| publisher |
Public Library of Science (PLoS) |
| publishDate |
2013 |
| url |
https://doaj.org/article/dd46bc48791b4ce0879fd71e2a336dcc |
| work_keys_str_mv |
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