Construction of a CRISPR/Cas9-Mediated Genome Editing System in Lentinula edodes
CRISPR/Cas9 genome editing systems have been established in a broad range of eukaryotic species. Herein, we report the first method for genetic engineering in pyogo (shiitake) mushrooms (Lentinula edodes) using CRISPR/Cas9. For in vivo expression of guide RNAs (gRNAs) targeting the mating-type gene...
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Autores principales: | , , , , , |
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Formato: | article |
Lenguaje: | EN |
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Taylor & Francis Group
2021
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Acceso en línea: | https://doaj.org/article/dd82cb282f1f469d879beef29d9a354d |
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