Construction of a CRISPR/Cas9-Mediated Genome Editing System in Lentinula edodes

CRISPR/Cas9 genome editing systems have been established in a broad range of eukaryotic species. Herein, we report the first method for genetic engineering in pyogo (shiitake) mushrooms (Lentinula edodes) using CRISPR/Cas9. For in vivo expression of guide RNAs (gRNAs) targeting the mating-type gene...

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Auteurs principaux: Suyun Moon, Jee Young An, Yeon-Jae Choi, Youn-Lee Oh, Hyeon-Su Ro, Hojin Ryu
Format: article
Langue:EN
Publié: Taylor & Francis Group 2021
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Accès en ligne:https://doaj.org/article/dd82cb282f1f469d879beef29d9a354d
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