Broad surveys of DNA viral diversity obtained through viral metagenomics of mosquitoes.

Viruses are the most abundant and diverse genetic entities on Earth; however, broad surveys of viral diversity are hindered by the lack of a universal assay for viruses and the inability to sample a sufficient number of individual hosts. This study utilized vector-enabled metagenomics (VEM) to provi...

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Autores principales:	Terry Fei Fan Ng, Dana L Willner, Yan Wei Lim, Robert Schmieder, Betty Chau, Christina Nilsson, Simon Anthony, Yijun Ruan, Forest Rohwer, Mya Breitbart
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/de0b5ed7994343e5a03c065339b75854
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spelling	oai:doaj.org-article:de0b5ed7994343e5a03c065339b758542021-11-18T06:52:33ZBroad surveys of DNA viral diversity obtained through viral metagenomics of mosquitoes.1932-620310.1371/journal.pone.0020579https://doaj.org/article/de0b5ed7994343e5a03c065339b758542011-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21674005/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Viruses are the most abundant and diverse genetic entities on Earth; however, broad surveys of viral diversity are hindered by the lack of a universal assay for viruses and the inability to sample a sufficient number of individual hosts. This study utilized vector-enabled metagenomics (VEM) to provide a snapshot of the diversity of DNA viruses present in three mosquito samples from San Diego, California. The majority of the sequences were novel, suggesting that the viral community in mosquitoes, as well as the animal and plant hosts they feed on, is highly diverse and largely uncharacterized. Each mosquito sample contained a distinct viral community. The mosquito viromes contained sequences related to a broad range of animal, plant, insect and bacterial viruses. Animal viruses identified included anelloviruses, circoviruses, herpesviruses, poxviruses, and papillomaviruses, which mosquitoes may have obtained from vertebrate hosts during blood feeding. Notably, sequences related to human papillomaviruses were identified in one of the mosquito samples. Sequences similar to plant viruses were identified in all mosquito viromes, which were potentially acquired through feeding on plant nectar. Numerous bacteriophages and insect viruses were also detected, including a novel densovirus likely infecting Culex erythrothorax. Through sampling insect vectors, VEM enables broad survey of viral diversity and has significantly increased our knowledge of the DNA viruses present in mosquitoes.Terry Fei Fan NgDana L WillnerYan Wei LimRobert SchmiederBetty ChauChristina NilssonSimon AnthonyYijun RuanForest RohwerMya BreitbartPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 6, p e20579 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Terry Fei Fan Ng Dana L Willner Yan Wei Lim Robert Schmieder Betty Chau Christina Nilsson Simon Anthony Yijun Ruan Forest Rohwer Mya Breitbart Broad surveys of DNA viral diversity obtained through viral metagenomics of mosquitoes.
description	Viruses are the most abundant and diverse genetic entities on Earth; however, broad surveys of viral diversity are hindered by the lack of a universal assay for viruses and the inability to sample a sufficient number of individual hosts. This study utilized vector-enabled metagenomics (VEM) to provide a snapshot of the diversity of DNA viruses present in three mosquito samples from San Diego, California. The majority of the sequences were novel, suggesting that the viral community in mosquitoes, as well as the animal and plant hosts they feed on, is highly diverse and largely uncharacterized. Each mosquito sample contained a distinct viral community. The mosquito viromes contained sequences related to a broad range of animal, plant, insect and bacterial viruses. Animal viruses identified included anelloviruses, circoviruses, herpesviruses, poxviruses, and papillomaviruses, which mosquitoes may have obtained from vertebrate hosts during blood feeding. Notably, sequences related to human papillomaviruses were identified in one of the mosquito samples. Sequences similar to plant viruses were identified in all mosquito viromes, which were potentially acquired through feeding on plant nectar. Numerous bacteriophages and insect viruses were also detected, including a novel densovirus likely infecting Culex erythrothorax. Through sampling insect vectors, VEM enables broad survey of viral diversity and has significantly increased our knowledge of the DNA viruses present in mosquitoes.
format	article
author	Terry Fei Fan Ng Dana L Willner Yan Wei Lim Robert Schmieder Betty Chau Christina Nilsson Simon Anthony Yijun Ruan Forest Rohwer Mya Breitbart
author_facet	Terry Fei Fan Ng Dana L Willner Yan Wei Lim Robert Schmieder Betty Chau Christina Nilsson Simon Anthony Yijun Ruan Forest Rohwer Mya Breitbart
author_sort	Terry Fei Fan Ng
title	Broad surveys of DNA viral diversity obtained through viral metagenomics of mosquitoes.
title_short	Broad surveys of DNA viral diversity obtained through viral metagenomics of mosquitoes.
title_full	Broad surveys of DNA viral diversity obtained through viral metagenomics of mosquitoes.
title_fullStr	Broad surveys of DNA viral diversity obtained through viral metagenomics of mosquitoes.
title_full_unstemmed	Broad surveys of DNA viral diversity obtained through viral metagenomics of mosquitoes.
title_sort	broad surveys of dna viral diversity obtained through viral metagenomics of mosquitoes.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/de0b5ed7994343e5a03c065339b75854
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Broad surveys of DNA viral diversity obtained through viral metagenomics of mosquitoes.

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