Real-space and real-time dynamics of CRISPR-Cas9 visualized by high-speed atomic force microscopy

CRISPR RNA-guided endonuclease Cas9 recognizes and cleaves the double-stranded DNA complementary to the RNA guide. Here the authors use high-speed atomic force micropcopy (HS-AFM) to visualize the conformational dynamics of Cas9 during its DNA targeting and cleavage processes.

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Autores principales:	Mikihiro Shibata, Hiroshi Nishimasu, Noriyuki Kodera, Seiichi Hirano, Toshio Ando, Takayuki Uchihashi, Osamu Nureki
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/de85d8052fbc4273b32ea84729525771
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spelling	oai:doaj.org-article:de85d8052fbc4273b32ea847295257712021-12-02T17:06:22ZReal-space and real-time dynamics of CRISPR-Cas9 visualized by high-speed atomic force microscopy10.1038/s41467-017-01466-82041-1723https://doaj.org/article/de85d8052fbc4273b32ea847295257712017-11-01T00:00:00Zhttps://doi.org/10.1038/s41467-017-01466-8https://doaj.org/toc/2041-1723CRISPR RNA-guided endonuclease Cas9 recognizes and cleaves the double-stranded DNA complementary to the RNA guide. Here the authors use high-speed atomic force micropcopy (HS-AFM) to visualize the conformational dynamics of Cas9 during its DNA targeting and cleavage processes.Mikihiro ShibataHiroshi NishimasuNoriyuki KoderaSeiichi HiranoToshio AndoTakayuki UchihashiOsamu NurekiNature PortfolioarticleScienceQENNature Communications, Vol 8, Iss 1, Pp 1-9 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Mikihiro Shibata Hiroshi Nishimasu Noriyuki Kodera Seiichi Hirano Toshio Ando Takayuki Uchihashi Osamu Nureki Real-space and real-time dynamics of CRISPR-Cas9 visualized by high-speed atomic force microscopy
description	CRISPR RNA-guided endonuclease Cas9 recognizes and cleaves the double-stranded DNA complementary to the RNA guide. Here the authors use high-speed atomic force micropcopy (HS-AFM) to visualize the conformational dynamics of Cas9 during its DNA targeting and cleavage processes.
format	article
author	Mikihiro Shibata Hiroshi Nishimasu Noriyuki Kodera Seiichi Hirano Toshio Ando Takayuki Uchihashi Osamu Nureki
author_facet	Mikihiro Shibata Hiroshi Nishimasu Noriyuki Kodera Seiichi Hirano Toshio Ando Takayuki Uchihashi Osamu Nureki
author_sort	Mikihiro Shibata
title	Real-space and real-time dynamics of CRISPR-Cas9 visualized by high-speed atomic force microscopy
title_short	Real-space and real-time dynamics of CRISPR-Cas9 visualized by high-speed atomic force microscopy
title_full	Real-space and real-time dynamics of CRISPR-Cas9 visualized by high-speed atomic force microscopy
title_fullStr	Real-space and real-time dynamics of CRISPR-Cas9 visualized by high-speed atomic force microscopy
title_full_unstemmed	Real-space and real-time dynamics of CRISPR-Cas9 visualized by high-speed atomic force microscopy
title_sort	real-space and real-time dynamics of crispr-cas9 visualized by high-speed atomic force microscopy
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/de85d8052fbc4273b32ea84729525771
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Real-space and real-time dynamics of CRISPR-Cas9 visualized by high-speed atomic force microscopy

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