Immunofluorescence microscopy-based detection of ssDNA foci by BrdU in mammalian cells

Summary: DNA end resection converts broken ends of double-stranded DNA (dsDNA) to 3′-single-stranded DNA (3′-ssDNA). The extent of resection regulates DNA double-strand break (DSB) repair pathway choice and thereby genomic stability. Here, we characterize an optimized immunofluorescence (IF) microsc...

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Autores principales: Susan Kilgas, Anne E. Kiltie, Kristijan Ramadan
Formato: article
Lenguaje:EN
Publicado: Elsevier 2021
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Acceso en línea:https://doaj.org/article/df88efb6bb6f41ffabbd89d98ecc3a22
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