Sprouty1 regulates gonadal white adipose tissue growth through a PDGFRα/β-Akt pathway

Expansion of visceral white adipose tissue (vWAT) occurs in response to nutrient excess, and is a risk factor for metabolic disease. SPRY1, a feedback inhibitor of receptor tyrosine kinase (RTK) signaling, is expressed in PDGFRa+ adipocyte progenitor cells (APC) in vivo. Global deficiency of Spry1 i...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Xuehui Yang, Shivangi Pande, Robert A. Koza, Robert Friesel
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2021
Materias:	adipocyte sprouty1 proliferation differentiation fibrosis cell signalling Diseases of the endocrine glands. Clinical endocrinology RC648-665 Cytology QH573-671 Physiology QP1-981
Acceso en línea:	https://doaj.org/article/dfd1f8d43a5c4d89876a7a582db6fd13
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:dfd1f8d43a5c4d89876a7a582db6fd13
record_format	dspace
spelling	oai:doaj.org-article:dfd1f8d43a5c4d89876a7a582db6fd132021-11-04T15:00:45ZSprouty1 regulates gonadal white adipose tissue growth through a PDGFRα/β-Akt pathway2162-39452162-397X10.1080/21623945.2021.1987634https://doaj.org/article/dfd1f8d43a5c4d89876a7a582db6fd132021-01-01T00:00:00Zhttp://dx.doi.org/10.1080/21623945.2021.1987634https://doaj.org/toc/2162-3945https://doaj.org/toc/2162-397XExpansion of visceral white adipose tissue (vWAT) occurs in response to nutrient excess, and is a risk factor for metabolic disease. SPRY1, a feedback inhibitor of receptor tyrosine kinase (RTK) signaling, is expressed in PDGFRa+ adipocyte progenitor cells (APC) in vivo. Global deficiency of Spry1 in mice results in disproportionate postnatal growth of gonadal WAT (gWAT), while iWAT and BAT were similar in size between Spry1KO and WT mice. Spry1 deficiency increased the number of PDGFRa+ stromal vascular fraction (SVF) cells in gWAT and showed increased proliferation and fibrosis. Spry1KO gWAT had increased collagen deposition and elevated expression of markers of inflammation. In vitro, SPRY1 was transiently down regulated during early adipocyte differentiation of SVF cells, with levels increasing at later stages of differentiation. SPRY1 deficiency enhances PDGF-AA and PDGF-BB induced proliferation of SVF cells. Increased proliferation of SVF from Spry1KO gWAT accompanies an increase in AKT activation. PDGF-AA stimulated a transient down regulation of SPRY1 in wild type SVF, whereas PDGF-BB stimulated a sustained down regulation of SPRY1 in wild type SVF. Collectively, our data suggest that SPRY1 is critical for regulating postnatal growth of gWAT by restraining APC proliferation and differentiation in part by regulation of PDGFRa/b-AKT signaling.Xuehui YangShivangi PandeRobert A. KozaRobert FrieselTaylor & Francis Grouparticleadipocytesprouty1proliferationdifferentiationfibrosiscell signallingDiseases of the endocrine glands. Clinical endocrinologyRC648-665CytologyQH573-671PhysiologyQP1-981ENAdipocyte, Vol 10, Iss 1, Pp 574-586 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	adipocyte sprouty1 proliferation differentiation fibrosis cell signalling Diseases of the endocrine glands. Clinical endocrinology RC648-665 Cytology QH573-671 Physiology QP1-981
spellingShingle	adipocyte sprouty1 proliferation differentiation fibrosis cell signalling Diseases of the endocrine glands. Clinical endocrinology RC648-665 Cytology QH573-671 Physiology QP1-981 Xuehui Yang Shivangi Pande Robert A. Koza Robert Friesel Sprouty1 regulates gonadal white adipose tissue growth through a PDGFRα/β-Akt pathway
description	Expansion of visceral white adipose tissue (vWAT) occurs in response to nutrient excess, and is a risk factor for metabolic disease. SPRY1, a feedback inhibitor of receptor tyrosine kinase (RTK) signaling, is expressed in PDGFRa+ adipocyte progenitor cells (APC) in vivo. Global deficiency of Spry1 in mice results in disproportionate postnatal growth of gonadal WAT (gWAT), while iWAT and BAT were similar in size between Spry1KO and WT mice. Spry1 deficiency increased the number of PDGFRa+ stromal vascular fraction (SVF) cells in gWAT and showed increased proliferation and fibrosis. Spry1KO gWAT had increased collagen deposition and elevated expression of markers of inflammation. In vitro, SPRY1 was transiently down regulated during early adipocyte differentiation of SVF cells, with levels increasing at later stages of differentiation. SPRY1 deficiency enhances PDGF-AA and PDGF-BB induced proliferation of SVF cells. Increased proliferation of SVF from Spry1KO gWAT accompanies an increase in AKT activation. PDGF-AA stimulated a transient down regulation of SPRY1 in wild type SVF, whereas PDGF-BB stimulated a sustained down regulation of SPRY1 in wild type SVF. Collectively, our data suggest that SPRY1 is critical for regulating postnatal growth of gWAT by restraining APC proliferation and differentiation in part by regulation of PDGFRa/b-AKT signaling.
format	article
author	Xuehui Yang Shivangi Pande Robert A. Koza Robert Friesel
author_facet	Xuehui Yang Shivangi Pande Robert A. Koza Robert Friesel
author_sort	Xuehui Yang
title	Sprouty1 regulates gonadal white adipose tissue growth through a PDGFRα/β-Akt pathway
title_short	Sprouty1 regulates gonadal white adipose tissue growth through a PDGFRα/β-Akt pathway
title_full	Sprouty1 regulates gonadal white adipose tissue growth through a PDGFRα/β-Akt pathway
title_fullStr	Sprouty1 regulates gonadal white adipose tissue growth through a PDGFRα/β-Akt pathway
title_full_unstemmed	Sprouty1 regulates gonadal white adipose tissue growth through a PDGFRα/β-Akt pathway
title_sort	sprouty1 regulates gonadal white adipose tissue growth through a pdgfrα/β-akt pathway
publisher	Taylor & Francis Group
publishDate	2021
url	https://doaj.org/article/dfd1f8d43a5c4d89876a7a582db6fd13
work_keys_str_mv	AT xuehuiyang sprouty1regulatesgonadalwhiteadiposetissuegrowththroughapdgfrabaktpathway AT shivangipande sprouty1regulatesgonadalwhiteadiposetissuegrowththroughapdgfrabaktpathway AT robertakoza sprouty1regulatesgonadalwhiteadiposetissuegrowththroughapdgfrabaktpathway AT robertfriesel sprouty1regulatesgonadalwhiteadiposetissuegrowththroughapdgfrabaktpathway
_version_	1718444803384934400

Sprouty1 regulates gonadal white adipose tissue growth through a PDGFRα/β-Akt pathway

Ejemplares similares