SPECT/CT imaging of hNIS-expression after intravenous delivery of an oncolytic adenovirus and 131I.
Oncolytic adenoviruses can be engineered for better tumor selectivity, gene delivery and be armed for imaging and concentrating radionuclides into tumors for synergistic oncolysis. We constructed Ad5/3-hTERT-hNIS where replication is controlled by hTERT-promoter. Ad5/3-hTERT-hNIS expresses hNIS for...
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2012
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oai:doaj.org-article:e17cf8fdf1f44f05a7bd565386ab942e2021-11-18T07:25:50ZSPECT/CT imaging of hNIS-expression after intravenous delivery of an oncolytic adenovirus and 131I.1932-620310.1371/journal.pone.0032871https://doaj.org/article/e17cf8fdf1f44f05a7bd565386ab942e2012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22412937/?tool=EBIhttps://doaj.org/toc/1932-6203Oncolytic adenoviruses can be engineered for better tumor selectivity, gene delivery and be armed for imaging and concentrating radionuclides into tumors for synergistic oncolysis. We constructed Ad5/3-hTERT-hNIS where replication is controlled by hTERT-promoter. Ad5/3-hTERT-hNIS expresses hNIS for imaging of transgene expression and for treatment of infected tumors by radioiodine. Ad5/3-hTERT-hNIS efficiently killed prostate cancer cells and induced iodine uptake in vitro and in vivo after intratumoral virus administration. Survival of mice treated with intravenous Ad5/3-hTERT-hNIS significantly prolonged survival over mock or radioiodine only but the combination of virus with radioiodine was not more effective than virus alone. Temporal and spatial changes in hNIS-expression during therapy were detected with SPECT, demonstrating feasibility of evaluation of the combination therapy with hNIS-expressing adenoviruses and radioiodide.Maria RajeckiMirkka SarparantaTanja HakkarainenMikko TenhunenIulia DiaconuVenla KuhmonenKalevi KairemoAnna KanervaAnu J AiraksinenAkseli HemminkiPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 3, p e32871 (2012) |
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Medicine R Science Q Maria Rajecki Mirkka Sarparanta Tanja Hakkarainen Mikko Tenhunen Iulia Diaconu Venla Kuhmonen Kalevi Kairemo Anna Kanerva Anu J Airaksinen Akseli Hemminki SPECT/CT imaging of hNIS-expression after intravenous delivery of an oncolytic adenovirus and 131I. |
| description |
Oncolytic adenoviruses can be engineered for better tumor selectivity, gene delivery and be armed for imaging and concentrating radionuclides into tumors for synergistic oncolysis. We constructed Ad5/3-hTERT-hNIS where replication is controlled by hTERT-promoter. Ad5/3-hTERT-hNIS expresses hNIS for imaging of transgene expression and for treatment of infected tumors by radioiodine. Ad5/3-hTERT-hNIS efficiently killed prostate cancer cells and induced iodine uptake in vitro and in vivo after intratumoral virus administration. Survival of mice treated with intravenous Ad5/3-hTERT-hNIS significantly prolonged survival over mock or radioiodine only but the combination of virus with radioiodine was not more effective than virus alone. Temporal and spatial changes in hNIS-expression during therapy were detected with SPECT, demonstrating feasibility of evaluation of the combination therapy with hNIS-expressing adenoviruses and radioiodide. |
| format |
article |
| author |
Maria Rajecki Mirkka Sarparanta Tanja Hakkarainen Mikko Tenhunen Iulia Diaconu Venla Kuhmonen Kalevi Kairemo Anna Kanerva Anu J Airaksinen Akseli Hemminki |
| author_facet |
Maria Rajecki Mirkka Sarparanta Tanja Hakkarainen Mikko Tenhunen Iulia Diaconu Venla Kuhmonen Kalevi Kairemo Anna Kanerva Anu J Airaksinen Akseli Hemminki |
| author_sort |
Maria Rajecki |
| title |
SPECT/CT imaging of hNIS-expression after intravenous delivery of an oncolytic adenovirus and 131I. |
| title_short |
SPECT/CT imaging of hNIS-expression after intravenous delivery of an oncolytic adenovirus and 131I. |
| title_full |
SPECT/CT imaging of hNIS-expression after intravenous delivery of an oncolytic adenovirus and 131I. |
| title_fullStr |
SPECT/CT imaging of hNIS-expression after intravenous delivery of an oncolytic adenovirus and 131I. |
| title_full_unstemmed |
SPECT/CT imaging of hNIS-expression after intravenous delivery of an oncolytic adenovirus and 131I. |
| title_sort |
spect/ct imaging of hnis-expression after intravenous delivery of an oncolytic adenovirus and 131i. |
| publisher |
Public Library of Science (PLoS) |
| publishDate |
2012 |
| url |
https://doaj.org/article/e17cf8fdf1f44f05a7bd565386ab942e |
| work_keys_str_mv |
AT mariarajecki spectctimagingofhnisexpressionafterintravenousdeliveryofanoncolyticadenovirusand131i AT mirkkasarparanta spectctimagingofhnisexpressionafterintravenousdeliveryofanoncolyticadenovirusand131i AT tanjahakkarainen spectctimagingofhnisexpressionafterintravenousdeliveryofanoncolyticadenovirusand131i AT mikkotenhunen spectctimagingofhnisexpressionafterintravenousdeliveryofanoncolyticadenovirusand131i AT iuliadiaconu spectctimagingofhnisexpressionafterintravenousdeliveryofanoncolyticadenovirusand131i AT venlakuhmonen spectctimagingofhnisexpressionafterintravenousdeliveryofanoncolyticadenovirusand131i AT kalevikairemo spectctimagingofhnisexpressionafterintravenousdeliveryofanoncolyticadenovirusand131i AT annakanerva spectctimagingofhnisexpressionafterintravenousdeliveryofanoncolyticadenovirusand131i AT anujairaksinen spectctimagingofhnisexpressionafterintravenousdeliveryofanoncolyticadenovirusand131i AT akselihemminki spectctimagingofhnisexpressionafterintravenousdeliveryofanoncolyticadenovirusand131i |
| _version_ |
1718423504685105152 |