Bacteria producing L-asparaginase isolated from Peruvian saline environments

L-asparaginase (EC 3.5.1.1) hydrolyzes L-asparagine in L-aspartic acid and ammonia. Its efficiency is subject to its kinetics and specificity on the substrate, characteristics that vary from one source to another. Thus, microorganisms from saline environments constitute a phylogenetic and metabolica...

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Autores principales:	Jeanett Montes, Pamela Elizabeth Canales, Juan Carlos Flores-Santos, Stephy Saavedra, Abad Hurtado-Gómez, Cynthia Esquerre-Huallpa, Amparo Iris Zavaleta
Formato:	article
Lenguaje:	ES
Publicado:	Universidad Nacional de Tumbes 2021
Materias:	Ecology QH540-549.5 Agriculture (General) S1-972 Plant culture SB1-1110 Animal culture SF1-1100
Acceso en línea:	https://doaj.org/article/e23ffd52dee049d2a012c16b247e57c7
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spelling	oai:doaj.org-article:e23ffd52dee049d2a012c16b247e57c72021-11-07T08:36:03ZBacteria producing L-asparaginase isolated from Peruvian saline environments10.17268/manglar.2021.0261816-76672414-1046https://doaj.org/article/e23ffd52dee049d2a012c16b247e57c72021-06-01T00:00:00Zhttps://erp.untumbes.edu.pe/revistas/index.php/manglar/article/view/243https://doaj.org/toc/1816-7667https://doaj.org/toc/2414-1046L-asparaginase (EC 3.5.1.1) hydrolyzes L-asparagine in L-aspartic acid and ammonia. Its efficiency is subject to its kinetics and specificity on the substrate, characteristics that vary from one source to another. Thus, microorganisms from saline environments constitute a phylogenetic and metabolically heterogeneous group for the search of new enzymes. Therefore, the objective of this study was the phenotypic and genotypic characterization of bacteria with L-asparaginase activity isolated from Maras, Pilluana and Chilca salterns. The 24 evaluated bacteria were classified as 38% Gram-negative bacilli and 54% positive; and 8% Gram-positive cocci. The majority grew in 5% salt water, pH 7.0 and 37 °C, all assimilated glucose. Of the 24 bacteria that produced L-asparaginase in solid medium, enzymatic activity was determined in submerged cultures by the Nessler method in 14 of them. The CH11, M62, M64, M68, and P19 strains identified as Bacillus sp., by partial sequencing of the 16S ribosomal gene, presented higher L-asparaginase activity and instability due to the presence of proteases. Saline environments bacteria are potential sources for the prospective production of L-asparaginase to use them as a therapeutic agent and in the food industry.Jeanett MontesPamela Elizabeth CanalesJuan Carlos Flores-SantosStephy SaavedraAbad Hurtado-GómezCynthia Esquerre-HuallpaAmparo Iris ZavaletaUniversidad Nacional de TumbesarticleEcologyQH540-549.5Agriculture (General)S1-972Plant cultureSB1-1110Animal cultureSF1-1100ESManglar, Vol 18, Iss 2, Pp 193-199 (2021)
institution	DOAJ
collection	DOAJ
language	ES
topic	Ecology QH540-549.5 Agriculture (General) S1-972 Plant culture SB1-1110 Animal culture SF1-1100
spellingShingle	Ecology QH540-549.5 Agriculture (General) S1-972 Plant culture SB1-1110 Animal culture SF1-1100 Jeanett Montes Pamela Elizabeth Canales Juan Carlos Flores-Santos Stephy Saavedra Abad Hurtado-Gómez Cynthia Esquerre-Huallpa Amparo Iris Zavaleta Bacteria producing L-asparaginase isolated from Peruvian saline environments
description	L-asparaginase (EC 3.5.1.1) hydrolyzes L-asparagine in L-aspartic acid and ammonia. Its efficiency is subject to its kinetics and specificity on the substrate, characteristics that vary from one source to another. Thus, microorganisms from saline environments constitute a phylogenetic and metabolically heterogeneous group for the search of new enzymes. Therefore, the objective of this study was the phenotypic and genotypic characterization of bacteria with L-asparaginase activity isolated from Maras, Pilluana and Chilca salterns. The 24 evaluated bacteria were classified as 38% Gram-negative bacilli and 54% positive; and 8% Gram-positive cocci. The majority grew in 5% salt water, pH 7.0 and 37 °C, all assimilated glucose. Of the 24 bacteria that produced L-asparaginase in solid medium, enzymatic activity was determined in submerged cultures by the Nessler method in 14 of them. The CH11, M62, M64, M68, and P19 strains identified as Bacillus sp., by partial sequencing of the 16S ribosomal gene, presented higher L-asparaginase activity and instability due to the presence of proteases. Saline environments bacteria are potential sources for the prospective production of L-asparaginase to use them as a therapeutic agent and in the food industry.
format	article
author	Jeanett Montes Pamela Elizabeth Canales Juan Carlos Flores-Santos Stephy Saavedra Abad Hurtado-Gómez Cynthia Esquerre-Huallpa Amparo Iris Zavaleta
author_facet	Jeanett Montes Pamela Elizabeth Canales Juan Carlos Flores-Santos Stephy Saavedra Abad Hurtado-Gómez Cynthia Esquerre-Huallpa Amparo Iris Zavaleta
author_sort	Jeanett Montes
title	Bacteria producing L-asparaginase isolated from Peruvian saline environments
title_short	Bacteria producing L-asparaginase isolated from Peruvian saline environments
title_full	Bacteria producing L-asparaginase isolated from Peruvian saline environments
title_fullStr	Bacteria producing L-asparaginase isolated from Peruvian saline environments
title_full_unstemmed	Bacteria producing L-asparaginase isolated from Peruvian saline environments
title_sort	bacteria producing l-asparaginase isolated from peruvian saline environments
publisher	Universidad Nacional de Tumbes
publishDate	2021
url	https://doaj.org/article/e23ffd52dee049d2a012c16b247e57c7
work_keys_str_mv	AT jeanettmontes bacteriaproducinglasparaginaseisolatedfromperuviansalineenvironments AT pamelaelizabethcanales bacteriaproducinglasparaginaseisolatedfromperuviansalineenvironments AT juancarlosfloressantos bacteriaproducinglasparaginaseisolatedfromperuviansalineenvironments AT stephysaavedra bacteriaproducinglasparaginaseisolatedfromperuviansalineenvironments AT abadhurtadogomez bacteriaproducinglasparaginaseisolatedfromperuviansalineenvironments AT cynthiaesquerrehuallpa bacteriaproducinglasparaginaseisolatedfromperuviansalineenvironments AT amparoiriszavaleta bacteriaproducinglasparaginaseisolatedfromperuviansalineenvironments
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Bacteria producing L-asparaginase isolated from Peruvian saline environments

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