Control of transcription by cell size.

Cell size increases significantly with increasing ploidy. Differences in cell size and ploidy are associated with alterations in gene expression, although no direct connection has been made between cell size and transcription. Here we show that ploidy-associated changes in gene expression reflect tr...

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Autores principales:	Chia-Yung Wu, P Alexander Rolfe, David K Gifford, Gerald R Fink
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2010
Materias:	Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/e24f1aac247e4af5b9273cb27dd26175
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spelling	oai:doaj.org-article:e24f1aac247e4af5b9273cb27dd261752021-11-18T05:36:27ZControl of transcription by cell size.1544-91731545-788510.1371/journal.pbio.1000523https://doaj.org/article/e24f1aac247e4af5b9273cb27dd261752010-11-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21072241/?tool=EBIhttps://doaj.org/toc/1544-9173https://doaj.org/toc/1545-7885Cell size increases significantly with increasing ploidy. Differences in cell size and ploidy are associated with alterations in gene expression, although no direct connection has been made between cell size and transcription. Here we show that ploidy-associated changes in gene expression reflect transcriptional adjustment to a larger cell size, implicating cellular geometry as a key parameter in gene regulation. Using RNA-seq, we identified genes whose expression was altered in a tetraploid as compared with the isogenic haploid. A significant fraction of these genes encode cell surface proteins, suggesting an effect of the enlarged cell size on the differential regulation of these genes. To test this hypothesis, we examined expression of these genes in haploid mutants that also produce enlarged size. Surprisingly, many genes differentially regulated in the tetraploid are identically regulated in the enlarged haploids, and the magnitude of change in gene expression correlates with the degree of size enlargement. These results indicate a causal relationship between cell size and transcription, with a size-sensing mechanism that alters transcription in response to size. The genes responding to cell size are enriched for those regulated by two mitogen-activated protein kinase pathways, and components in those pathways were found to mediate size-dependent gene regulation. Transcriptional adjustment to enlarged cell size could underlie other cellular changes associated with polyploidy. The causal relationship between cell size and transcription suggests that cell size homeostasis serves a regulatory role in transcriptome maintenance.Chia-Yung WuP Alexander RolfeDavid K GiffordGerald R FinkPublic Library of Science (PLoS)articleBiology (General)QH301-705.5ENPLoS Biology, Vol 8, Iss 11, p e1000523 (2010)
institution	DOAJ
collection	DOAJ
language	EN
topic	Biology (General) QH301-705.5
spellingShingle	Biology (General) QH301-705.5 Chia-Yung Wu P Alexander Rolfe David K Gifford Gerald R Fink Control of transcription by cell size.
description	Cell size increases significantly with increasing ploidy. Differences in cell size and ploidy are associated with alterations in gene expression, although no direct connection has been made between cell size and transcription. Here we show that ploidy-associated changes in gene expression reflect transcriptional adjustment to a larger cell size, implicating cellular geometry as a key parameter in gene regulation. Using RNA-seq, we identified genes whose expression was altered in a tetraploid as compared with the isogenic haploid. A significant fraction of these genes encode cell surface proteins, suggesting an effect of the enlarged cell size on the differential regulation of these genes. To test this hypothesis, we examined expression of these genes in haploid mutants that also produce enlarged size. Surprisingly, many genes differentially regulated in the tetraploid are identically regulated in the enlarged haploids, and the magnitude of change in gene expression correlates with the degree of size enlargement. These results indicate a causal relationship between cell size and transcription, with a size-sensing mechanism that alters transcription in response to size. The genes responding to cell size are enriched for those regulated by two mitogen-activated protein kinase pathways, and components in those pathways were found to mediate size-dependent gene regulation. Transcriptional adjustment to enlarged cell size could underlie other cellular changes associated with polyploidy. The causal relationship between cell size and transcription suggests that cell size homeostasis serves a regulatory role in transcriptome maintenance.
format	article
author	Chia-Yung Wu P Alexander Rolfe David K Gifford Gerald R Fink
author_facet	Chia-Yung Wu P Alexander Rolfe David K Gifford Gerald R Fink
author_sort	Chia-Yung Wu
title	Control of transcription by cell size.
title_short	Control of transcription by cell size.
title_full	Control of transcription by cell size.
title_fullStr	Control of transcription by cell size.
title_full_unstemmed	Control of transcription by cell size.
title_sort	control of transcription by cell size.
publisher	Public Library of Science (PLoS)
publishDate	2010
url	https://doaj.org/article/e24f1aac247e4af5b9273cb27dd26175
work_keys_str_mv	AT chiayungwu controloftranscriptionbycellsize AT palexanderrolfe controloftranscriptionbycellsize AT davidkgifford controloftranscriptionbycellsize AT geraldrfink controloftranscriptionbycellsize
_version_	1718424945516609536

Control of transcription by cell size.

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