MiR-320a as a Potential Novel Circulating Biomarker of Arrhythmogenic CardioMyopathy
Abstract Diagnosis of Arrhythmogenic CardioMyopathy (ACM) is challenging and often late after disease onset. No circulating biomarkers are available to date. Given their involvement in several cardiovascular diseases, plasma microRNAs warranted investigation as potential non-invasive diagnostic tool...
Guardado en:
| Autores principales: | , , , , , , , , , , , , , , , , , , , , , , , |
|---|---|
| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
Nature Portfolio
2017
|
| Materias: | |
| Acceso en línea: | https://doaj.org/article/e26e274e01914cf2a070af4986535dc0 |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| id |
oai:doaj.org-article:e26e274e01914cf2a070af4986535dc0 |
|---|---|
| record_format |
dspace |
| spelling |
oai:doaj.org-article:e26e274e01914cf2a070af4986535dc02021-12-02T12:30:25ZMiR-320a as a Potential Novel Circulating Biomarker of Arrhythmogenic CardioMyopathy10.1038/s41598-017-05001-z2045-2322https://doaj.org/article/e26e274e01914cf2a070af4986535dc02017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-05001-zhttps://doaj.org/toc/2045-2322Abstract Diagnosis of Arrhythmogenic CardioMyopathy (ACM) is challenging and often late after disease onset. No circulating biomarkers are available to date. Given their involvement in several cardiovascular diseases, plasma microRNAs warranted investigation as potential non-invasive diagnostic tools in ACM. We sought to identify circulating microRNAs differentially expressed in ACM with respect to Healthy Controls (HC) and Idiopathic Ventricular Tachycardia patients (IVT), often in differential diagnosis. ACM and HC subjects were screened for plasmatic expression of 377 microRNAs and validation was performed in 36 ACM, 53 HC, 21 IVT. Variable importance in data partition was estimated through Random Forest analysis and accuracy by Receiver Operating Curves. Plasmatic miR-320a showed 0.53 ± 0.04 fold expression difference in ACM vs. HC (p < 0.01). A similar trend was observed when comparing ACM (n = 13) and HC (n = 17) with athletic lifestyle, a ACM precipitating factor. Importantly, ACM patients miR-320a showed 0.78 ± 0.05 fold expression change vs. IVT (p = 0.03). When compared to non-invasive ACM diagnostic parameters, miR-320a ranked highly in discriminating ACM vs. IVT and it increased their accuracy. Finally, miR-320a expression did not correlate with ACM severity. Our data suggest that miR-320a may be considered a novel potential biomarker of ACM, specifically useful in ACM vs. IVT differentiation.Elena SommarivaYuri D’AlessandraFloriana Maria FarinaMichela CasellaFabio CattaneoValentina CattoMattia ChiesaIlaria StadiottiSilvia BrambillaAntonio Dello RussoCorrado CarbucicchioGiulia VettorDaniela RiggioMaria Teresa SandriAndrea BarbutiGianluca VernilloManuela MuratoriMatteo Dal FerroGianfranco SinagraSilvia MoimasMauro GiaccaGualtiero Ivanoe ColomboGiulio PompilioClaudio TondoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-10 (2017) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
Medicine R Science Q |
| spellingShingle |
Medicine R Science Q Elena Sommariva Yuri D’Alessandra Floriana Maria Farina Michela Casella Fabio Cattaneo Valentina Catto Mattia Chiesa Ilaria Stadiotti Silvia Brambilla Antonio Dello Russo Corrado Carbucicchio Giulia Vettor Daniela Riggio Maria Teresa Sandri Andrea Barbuti Gianluca Vernillo Manuela Muratori Matteo Dal Ferro Gianfranco Sinagra Silvia Moimas Mauro Giacca Gualtiero Ivanoe Colombo Giulio Pompilio Claudio Tondo MiR-320a as a Potential Novel Circulating Biomarker of Arrhythmogenic CardioMyopathy |
| description |
Abstract Diagnosis of Arrhythmogenic CardioMyopathy (ACM) is challenging and often late after disease onset. No circulating biomarkers are available to date. Given their involvement in several cardiovascular diseases, plasma microRNAs warranted investigation as potential non-invasive diagnostic tools in ACM. We sought to identify circulating microRNAs differentially expressed in ACM with respect to Healthy Controls (HC) and Idiopathic Ventricular Tachycardia patients (IVT), often in differential diagnosis. ACM and HC subjects were screened for plasmatic expression of 377 microRNAs and validation was performed in 36 ACM, 53 HC, 21 IVT. Variable importance in data partition was estimated through Random Forest analysis and accuracy by Receiver Operating Curves. Plasmatic miR-320a showed 0.53 ± 0.04 fold expression difference in ACM vs. HC (p < 0.01). A similar trend was observed when comparing ACM (n = 13) and HC (n = 17) with athletic lifestyle, a ACM precipitating factor. Importantly, ACM patients miR-320a showed 0.78 ± 0.05 fold expression change vs. IVT (p = 0.03). When compared to non-invasive ACM diagnostic parameters, miR-320a ranked highly in discriminating ACM vs. IVT and it increased their accuracy. Finally, miR-320a expression did not correlate with ACM severity. Our data suggest that miR-320a may be considered a novel potential biomarker of ACM, specifically useful in ACM vs. IVT differentiation. |
| format |
article |
| author |
Elena Sommariva Yuri D’Alessandra Floriana Maria Farina Michela Casella Fabio Cattaneo Valentina Catto Mattia Chiesa Ilaria Stadiotti Silvia Brambilla Antonio Dello Russo Corrado Carbucicchio Giulia Vettor Daniela Riggio Maria Teresa Sandri Andrea Barbuti Gianluca Vernillo Manuela Muratori Matteo Dal Ferro Gianfranco Sinagra Silvia Moimas Mauro Giacca Gualtiero Ivanoe Colombo Giulio Pompilio Claudio Tondo |
| author_facet |
Elena Sommariva Yuri D’Alessandra Floriana Maria Farina Michela Casella Fabio Cattaneo Valentina Catto Mattia Chiesa Ilaria Stadiotti Silvia Brambilla Antonio Dello Russo Corrado Carbucicchio Giulia Vettor Daniela Riggio Maria Teresa Sandri Andrea Barbuti Gianluca Vernillo Manuela Muratori Matteo Dal Ferro Gianfranco Sinagra Silvia Moimas Mauro Giacca Gualtiero Ivanoe Colombo Giulio Pompilio Claudio Tondo |
| author_sort |
Elena Sommariva |
| title |
MiR-320a as a Potential Novel Circulating Biomarker of Arrhythmogenic CardioMyopathy |
| title_short |
MiR-320a as a Potential Novel Circulating Biomarker of Arrhythmogenic CardioMyopathy |
| title_full |
MiR-320a as a Potential Novel Circulating Biomarker of Arrhythmogenic CardioMyopathy |
| title_fullStr |
MiR-320a as a Potential Novel Circulating Biomarker of Arrhythmogenic CardioMyopathy |
| title_full_unstemmed |
MiR-320a as a Potential Novel Circulating Biomarker of Arrhythmogenic CardioMyopathy |
| title_sort |
mir-320a as a potential novel circulating biomarker of arrhythmogenic cardiomyopathy |
| publisher |
Nature Portfolio |
| publishDate |
2017 |
| url |
https://doaj.org/article/e26e274e01914cf2a070af4986535dc0 |
| work_keys_str_mv |
AT elenasommariva mir320aasapotentialnovelcirculatingbiomarkerofarrhythmogeniccardiomyopathy AT yuridalessandra mir320aasapotentialnovelcirculatingbiomarkerofarrhythmogeniccardiomyopathy AT florianamariafarina mir320aasapotentialnovelcirculatingbiomarkerofarrhythmogeniccardiomyopathy AT michelacasella mir320aasapotentialnovelcirculatingbiomarkerofarrhythmogeniccardiomyopathy AT fabiocattaneo mir320aasapotentialnovelcirculatingbiomarkerofarrhythmogeniccardiomyopathy AT valentinacatto mir320aasapotentialnovelcirculatingbiomarkerofarrhythmogeniccardiomyopathy AT mattiachiesa mir320aasapotentialnovelcirculatingbiomarkerofarrhythmogeniccardiomyopathy AT ilariastadiotti mir320aasapotentialnovelcirculatingbiomarkerofarrhythmogeniccardiomyopathy AT silviabrambilla mir320aasapotentialnovelcirculatingbiomarkerofarrhythmogeniccardiomyopathy AT antoniodellorusso mir320aasapotentialnovelcirculatingbiomarkerofarrhythmogeniccardiomyopathy AT corradocarbucicchio mir320aasapotentialnovelcirculatingbiomarkerofarrhythmogeniccardiomyopathy AT giuliavettor mir320aasapotentialnovelcirculatingbiomarkerofarrhythmogeniccardiomyopathy AT danielariggio mir320aasapotentialnovelcirculatingbiomarkerofarrhythmogeniccardiomyopathy AT mariateresasandri mir320aasapotentialnovelcirculatingbiomarkerofarrhythmogeniccardiomyopathy AT andreabarbuti mir320aasapotentialnovelcirculatingbiomarkerofarrhythmogeniccardiomyopathy AT gianlucavernillo mir320aasapotentialnovelcirculatingbiomarkerofarrhythmogeniccardiomyopathy AT manuelamuratori mir320aasapotentialnovelcirculatingbiomarkerofarrhythmogeniccardiomyopathy AT matteodalferro mir320aasapotentialnovelcirculatingbiomarkerofarrhythmogeniccardiomyopathy AT gianfrancosinagra mir320aasapotentialnovelcirculatingbiomarkerofarrhythmogeniccardiomyopathy AT silviamoimas mir320aasapotentialnovelcirculatingbiomarkerofarrhythmogeniccardiomyopathy AT maurogiacca mir320aasapotentialnovelcirculatingbiomarkerofarrhythmogeniccardiomyopathy AT gualtieroivanoecolombo mir320aasapotentialnovelcirculatingbiomarkerofarrhythmogeniccardiomyopathy AT giuliopompilio mir320aasapotentialnovelcirculatingbiomarkerofarrhythmogeniccardiomyopathy AT claudiotondo mir320aasapotentialnovelcirculatingbiomarkerofarrhythmogeniccardiomyopathy |
| _version_ |
1718394428983345152 |