Detection of EGFR mutation in plasma using multiplex allele-specific PCR (MAS-PCR) and surface enhanced Raman spectroscopy

Abstract In this study, surface enhanced Raman spectroscopy (SERS) in combination with multiplexed polymerase chain reaction (PCR) was utilized to detect mutations of exons 19 and 21 of the epidermal growth factor receptor (EGFR) gene. Through the use of multiplexed PCR, the two mutation types were...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Xiaozhou Li, Tianyue Yang, Caesar Siqi Li, Deli Wang, Youtao Song, Lili Jin
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/e3dd0c1ae82446f2aade50835824509f
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:e3dd0c1ae82446f2aade50835824509f
record_format	dspace
spelling	oai:doaj.org-article:e3dd0c1ae82446f2aade50835824509f2021-12-02T16:05:59ZDetection of EGFR mutation in plasma using multiplex allele-specific PCR (MAS-PCR) and surface enhanced Raman spectroscopy10.1038/s41598-017-05050-42045-2322https://doaj.org/article/e3dd0c1ae82446f2aade50835824509f2017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-05050-4https://doaj.org/toc/2045-2322Abstract In this study, surface enhanced Raman spectroscopy (SERS) in combination with multiplexed polymerase chain reaction (PCR) was utilized to detect mutations of exons 19 and 21 of the epidermal growth factor receptor (EGFR) gene. Through the use of multiplexed PCR, the two mutation types were amplified in a single reaction. SERS was used on the PCR products to detect mutations. DNA mixtures with increasing mutation percentages showed good linear relationship between mutation rates and peak height. Then, this PCR-SERS method was used on the plasma of 48 patients with non-small cell lung cancer (NSCLC) to detect EGFR mutations. Analysis of variance (ANOVA) and receiver operating characteristic (ROC) analysis revealed that the peak height ratios were significant for identifying different mutation types. The specificity, sensitivity and accuracy obtained were all 100%. The proposed method was then validated through comparison with high resolution melting (HRM) and showed high concordance with HRM (Pearson correlation is 0.92). Finally, logistic regression was performed on EGFR mutation status and the clinical features of the 48 patients. Our study indicates that PCR-SERS is an effective, noninvasive, and economical method for the detection and monitoring of EGFR mutations in the plasma of patients with NSCLC.Xiaozhou LiTianyue YangCaesar Siqi LiDeli WangYoutao SongLili JinNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-9 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Xiaozhou Li Tianyue Yang Caesar Siqi Li Deli Wang Youtao Song Lili Jin Detection of EGFR mutation in plasma using multiplex allele-specific PCR (MAS-PCR) and surface enhanced Raman spectroscopy
description	Abstract In this study, surface enhanced Raman spectroscopy (SERS) in combination with multiplexed polymerase chain reaction (PCR) was utilized to detect mutations of exons 19 and 21 of the epidermal growth factor receptor (EGFR) gene. Through the use of multiplexed PCR, the two mutation types were amplified in a single reaction. SERS was used on the PCR products to detect mutations. DNA mixtures with increasing mutation percentages showed good linear relationship between mutation rates and peak height. Then, this PCR-SERS method was used on the plasma of 48 patients with non-small cell lung cancer (NSCLC) to detect EGFR mutations. Analysis of variance (ANOVA) and receiver operating characteristic (ROC) analysis revealed that the peak height ratios were significant for identifying different mutation types. The specificity, sensitivity and accuracy obtained were all 100%. The proposed method was then validated through comparison with high resolution melting (HRM) and showed high concordance with HRM (Pearson correlation is 0.92). Finally, logistic regression was performed on EGFR mutation status and the clinical features of the 48 patients. Our study indicates that PCR-SERS is an effective, noninvasive, and economical method for the detection and monitoring of EGFR mutations in the plasma of patients with NSCLC.
format	article
author	Xiaozhou Li Tianyue Yang Caesar Siqi Li Deli Wang Youtao Song Lili Jin
author_facet	Xiaozhou Li Tianyue Yang Caesar Siqi Li Deli Wang Youtao Song Lili Jin
author_sort	Xiaozhou Li
title	Detection of EGFR mutation in plasma using multiplex allele-specific PCR (MAS-PCR) and surface enhanced Raman spectroscopy
title_short	Detection of EGFR mutation in plasma using multiplex allele-specific PCR (MAS-PCR) and surface enhanced Raman spectroscopy
title_full	Detection of EGFR mutation in plasma using multiplex allele-specific PCR (MAS-PCR) and surface enhanced Raman spectroscopy
title_fullStr	Detection of EGFR mutation in plasma using multiplex allele-specific PCR (MAS-PCR) and surface enhanced Raman spectroscopy
title_full_unstemmed	Detection of EGFR mutation in plasma using multiplex allele-specific PCR (MAS-PCR) and surface enhanced Raman spectroscopy
title_sort	detection of egfr mutation in plasma using multiplex allele-specific pcr (mas-pcr) and surface enhanced raman spectroscopy
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/e3dd0c1ae82446f2aade50835824509f
work_keys_str_mv	AT xiaozhouli detectionofegfrmutationinplasmausingmultiplexallelespecificpcrmaspcrandsurfaceenhancedramanspectroscopy AT tianyueyang detectionofegfrmutationinplasmausingmultiplexallelespecificpcrmaspcrandsurfaceenhancedramanspectroscopy AT caesarsiqili detectionofegfrmutationinplasmausingmultiplexallelespecificpcrmaspcrandsurfaceenhancedramanspectroscopy AT deliwang detectionofegfrmutationinplasmausingmultiplexallelespecificpcrmaspcrandsurfaceenhancedramanspectroscopy AT youtaosong detectionofegfrmutationinplasmausingmultiplexallelespecificpcrmaspcrandsurfaceenhancedramanspectroscopy AT lilijin detectionofegfrmutationinplasmausingmultiplexallelespecificpcrmaspcrandsurfaceenhancedramanspectroscopy
_version_	1718385180721283072

Detection of EGFR mutation in plasma using multiplex allele-specific PCR (MAS-PCR) and surface enhanced Raman spectroscopy

Ejemplares similares