Docosahexaenoic Acid and Eicosapentaenoic Acid Intakes Modulate the Association of <i>FADS2</i> Gene Polymorphism rs526126 with Plasma Free Docosahexaenoic Acid Levels in Overweight Children

Polyunsaturated fatty acids are involved in a wide variety of biological functions. Linoleic acid and alpha-linolenic acid are two essential fatty acids that the body cannot synthesize. The conversion rates in the body depend on <i>FADS2</i> genetic variants. Certain variations in this g...

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Autores principales:	Alexandra Mihailescu, Vlad Serafim, Corina Paul, Nicoleta Andreescu, Diana-Andreea Tiugan, Paul Tutac, Iulian Velea, Cristian G. Zimbru, Costela Lacrimioara Serban, Adina Iuliana Ion, Vlad Laurentiu David, Alin Ionescu, Maria Puiu, Mihai Dinu Niculescu
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	long chain polyunsaturated fatty acids (LC-PUFAs) <i>FADS2</i> gene pediatric obesity dietary intake Technology T Engineering (General). Civil engineering (General) TA1-2040 Biology (General) QH301-705.5 Physics QC1-999 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/e5418b276a28412aac556d54b9dc28c9
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spelling	oai:doaj.org-article:e5418b276a28412aac556d54b9dc28c92021-11-11T14:58:50ZDocosahexaenoic Acid and Eicosapentaenoic Acid Intakes Modulate the Association of <i>FADS2</i> Gene Polymorphism rs526126 with Plasma Free Docosahexaenoic Acid Levels in Overweight Children10.3390/app112198452076-3417https://doaj.org/article/e5418b276a28412aac556d54b9dc28c92021-10-01T00:00:00Zhttps://www.mdpi.com/2076-3417/11/21/9845https://doaj.org/toc/2076-3417Polyunsaturated fatty acids are involved in a wide variety of biological functions. Linoleic acid and alpha-linolenic acid are two essential fatty acids that the body cannot synthesize. The conversion rates in the body depend on <i>FADS2</i> genetic variants. Certain variations in this gene are directly responsible for the low levels and poor conversion efficiency of the delta-6 desaturase enzyme, resulting in low circulating levels of docosahexaenoic acid. In this study, we evaluated the impact of the rs526126 <i>FADS2</i> gene polymorphism on fatty acid levels in a group of two hundred children (<i>n</i> = 95 males, <i>n</i> = 105 females) aged 7–18 years, with obesity defined by BMI > +2 SD. Fatty acid quantification was performed by LC-MS/MS while genotyping for genetic variants was performed using a custom-made hotspot sequencing panel of 55 SNPs. Our results suggest that rs526126 <i>FADS2</i> gene polymorphism specifically impacts the plasma levels of free n-3 polyunsaturated fatty acids. Finally, the presence of the minor allele G of rs526126 could have beneficial effects, as it was associated with higher levels of free docosahexaenoic acid in plasma, especially in children with low n-3 intakes.Alexandra MihailescuVlad SerafimCorina PaulNicoleta AndreescuDiana-Andreea TiuganPaul TutacIulian VeleaCristian G. ZimbruCostela Lacrimioara SerbanAdina Iuliana IonVlad Laurentiu DavidAlin IonescuMaria PuiuMihai Dinu NiculescuMDPI AGarticlelong chain polyunsaturated fatty acids (LC-PUFAs)<i>FADS2</i> genepediatric obesitydietary intakeTechnologyTEngineering (General). Civil engineering (General)TA1-2040Biology (General)QH301-705.5PhysicsQC1-999ChemistryQD1-999ENApplied Sciences, Vol 11, Iss 9845, p 9845 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	long chain polyunsaturated fatty acids (LC-PUFAs) <i>FADS2</i> gene pediatric obesity dietary intake Technology T Engineering (General). Civil engineering (General) TA1-2040 Biology (General) QH301-705.5 Physics QC1-999 Chemistry QD1-999
spellingShingle	long chain polyunsaturated fatty acids (LC-PUFAs) <i>FADS2</i> gene pediatric obesity dietary intake Technology T Engineering (General). Civil engineering (General) TA1-2040 Biology (General) QH301-705.5 Physics QC1-999 Chemistry QD1-999 Alexandra Mihailescu Vlad Serafim Corina Paul Nicoleta Andreescu Diana-Andreea Tiugan Paul Tutac Iulian Velea Cristian G. Zimbru Costela Lacrimioara Serban Adina Iuliana Ion Vlad Laurentiu David Alin Ionescu Maria Puiu Mihai Dinu Niculescu Docosahexaenoic Acid and Eicosapentaenoic Acid Intakes Modulate the Association of <i>FADS2</i> Gene Polymorphism rs526126 with Plasma Free Docosahexaenoic Acid Levels in Overweight Children
description	Polyunsaturated fatty acids are involved in a wide variety of biological functions. Linoleic acid and alpha-linolenic acid are two essential fatty acids that the body cannot synthesize. The conversion rates in the body depend on <i>FADS2</i> genetic variants. Certain variations in this gene are directly responsible for the low levels and poor conversion efficiency of the delta-6 desaturase enzyme, resulting in low circulating levels of docosahexaenoic acid. In this study, we evaluated the impact of the rs526126 <i>FADS2</i> gene polymorphism on fatty acid levels in a group of two hundred children (<i>n</i> = 95 males, <i>n</i> = 105 females) aged 7–18 years, with obesity defined by BMI > +2 SD. Fatty acid quantification was performed by LC-MS/MS while genotyping for genetic variants was performed using a custom-made hotspot sequencing panel of 55 SNPs. Our results suggest that rs526126 <i>FADS2</i> gene polymorphism specifically impacts the plasma levels of free n-3 polyunsaturated fatty acids. Finally, the presence of the minor allele G of rs526126 could have beneficial effects, as it was associated with higher levels of free docosahexaenoic acid in plasma, especially in children with low n-3 intakes.
format	article
author	Alexandra Mihailescu Vlad Serafim Corina Paul Nicoleta Andreescu Diana-Andreea Tiugan Paul Tutac Iulian Velea Cristian G. Zimbru Costela Lacrimioara Serban Adina Iuliana Ion Vlad Laurentiu David Alin Ionescu Maria Puiu Mihai Dinu Niculescu
author_facet	Alexandra Mihailescu Vlad Serafim Corina Paul Nicoleta Andreescu Diana-Andreea Tiugan Paul Tutac Iulian Velea Cristian G. Zimbru Costela Lacrimioara Serban Adina Iuliana Ion Vlad Laurentiu David Alin Ionescu Maria Puiu Mihai Dinu Niculescu
author_sort	Alexandra Mihailescu
title	Docosahexaenoic Acid and Eicosapentaenoic Acid Intakes Modulate the Association of <i>FADS2</i> Gene Polymorphism rs526126 with Plasma Free Docosahexaenoic Acid Levels in Overweight Children
title_short	Docosahexaenoic Acid and Eicosapentaenoic Acid Intakes Modulate the Association of <i>FADS2</i> Gene Polymorphism rs526126 with Plasma Free Docosahexaenoic Acid Levels in Overweight Children
title_full	Docosahexaenoic Acid and Eicosapentaenoic Acid Intakes Modulate the Association of <i>FADS2</i> Gene Polymorphism rs526126 with Plasma Free Docosahexaenoic Acid Levels in Overweight Children
title_fullStr	Docosahexaenoic Acid and Eicosapentaenoic Acid Intakes Modulate the Association of <i>FADS2</i> Gene Polymorphism rs526126 with Plasma Free Docosahexaenoic Acid Levels in Overweight Children
title_full_unstemmed	Docosahexaenoic Acid and Eicosapentaenoic Acid Intakes Modulate the Association of <i>FADS2</i> Gene Polymorphism rs526126 with Plasma Free Docosahexaenoic Acid Levels in Overweight Children
title_sort	docosahexaenoic acid and eicosapentaenoic acid intakes modulate the association of <i>fads2</i> gene polymorphism rs526126 with plasma free docosahexaenoic acid levels in overweight children
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/e5418b276a28412aac556d54b9dc28c9
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Docosahexaenoic Acid and Eicosapentaenoic Acid Intakes Modulate the Association of <i>FADS2</i> Gene Polymorphism rs526126 with Plasma Free Docosahexaenoic Acid Levels in Overweight Children

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