Identification of germ cell-specific Mga variant mRNA that promotes meiosis via impediment of a non-canonical PRC1

Abstract A non-canonical PRC1 (PRC1.6) prevents precocious meiotic onset. Germ cells alleviate its negative effect by reducing their amount of MAX, a component of PRC1.6, as a prerequisite for their bona fide meiosis. Here, we found that germ cells produced Mga variant mRNA bearing a premature termi...

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Autores principales:	Yuka Kitamura, Kousuke Uranishi, Masataka Hirasaki, Masazumi Nishimoto, Ayumu Suzuki, Akihiko Okuda
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/e5725950dfaa4ffa90fb9e62a661d97f
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spelling	oai:doaj.org-article:e5725950dfaa4ffa90fb9e62a661d97f2021-12-02T14:49:43ZIdentification of germ cell-specific Mga variant mRNA that promotes meiosis via impediment of a non-canonical PRC110.1038/s41598-021-89123-52045-2322https://doaj.org/article/e5725950dfaa4ffa90fb9e62a661d97f2021-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-89123-5https://doaj.org/toc/2045-2322Abstract A non-canonical PRC1 (PRC1.6) prevents precocious meiotic onset. Germ cells alleviate its negative effect by reducing their amount of MAX, a component of PRC1.6, as a prerequisite for their bona fide meiosis. Here, we found that germ cells produced Mga variant mRNA bearing a premature termination codon (PTC) during meiosis as an additional mechanism to impede the function of PRC1.6. The variant mRNA encodes an anomalous MGA protein that lacks the bHLHZ domain and thus functions as a dominant negative regulator of PRC1.6. Notwithstanding the presence of PTC, the Mga variant mRNA are rather stably present in spermatocytes and spermatids due to their intrinsic inefficient background of nonsense-mediated mRNA decay. Thus, our data indicate that meiosis is controlled in a multi-layered manner in which both MAX and MGA, which constitute the core of PRC1.6, are at least used as targets to deteriorate the integrity of the complex to ensure progression of meiosis.Yuka KitamuraKousuke UranishiMasataka HirasakiMasazumi NishimotoAyumu SuzukiAkihiko OkudaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-14 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yuka Kitamura Kousuke Uranishi Masataka Hirasaki Masazumi Nishimoto Ayumu Suzuki Akihiko Okuda Identification of germ cell-specific Mga variant mRNA that promotes meiosis via impediment of a non-canonical PRC1
description	Abstract A non-canonical PRC1 (PRC1.6) prevents precocious meiotic onset. Germ cells alleviate its negative effect by reducing their amount of MAX, a component of PRC1.6, as a prerequisite for their bona fide meiosis. Here, we found that germ cells produced Mga variant mRNA bearing a premature termination codon (PTC) during meiosis as an additional mechanism to impede the function of PRC1.6. The variant mRNA encodes an anomalous MGA protein that lacks the bHLHZ domain and thus functions as a dominant negative regulator of PRC1.6. Notwithstanding the presence of PTC, the Mga variant mRNA are rather stably present in spermatocytes and spermatids due to their intrinsic inefficient background of nonsense-mediated mRNA decay. Thus, our data indicate that meiosis is controlled in a multi-layered manner in which both MAX and MGA, which constitute the core of PRC1.6, are at least used as targets to deteriorate the integrity of the complex to ensure progression of meiosis.
format	article
author	Yuka Kitamura Kousuke Uranishi Masataka Hirasaki Masazumi Nishimoto Ayumu Suzuki Akihiko Okuda
author_facet	Yuka Kitamura Kousuke Uranishi Masataka Hirasaki Masazumi Nishimoto Ayumu Suzuki Akihiko Okuda
author_sort	Yuka Kitamura
title	Identification of germ cell-specific Mga variant mRNA that promotes meiosis via impediment of a non-canonical PRC1
title_short	Identification of germ cell-specific Mga variant mRNA that promotes meiosis via impediment of a non-canonical PRC1
title_full	Identification of germ cell-specific Mga variant mRNA that promotes meiosis via impediment of a non-canonical PRC1
title_fullStr	Identification of germ cell-specific Mga variant mRNA that promotes meiosis via impediment of a non-canonical PRC1
title_full_unstemmed	Identification of germ cell-specific Mga variant mRNA that promotes meiosis via impediment of a non-canonical PRC1
title_sort	identification of germ cell-specific mga variant mrna that promotes meiosis via impediment of a non-canonical prc1
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/e5725950dfaa4ffa90fb9e62a661d97f
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Identification of germ cell-specific Mga variant mRNA that promotes meiosis via impediment of a non-canonical PRC1

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