Programming Native CRISPR Arrays for the Generation of Targeted Immunity

ABSTRACT The adaptive immune system of prokaryotes, called CRISPR-Cas (clustered regularly interspaced short palindromic repeats and CRISPR-associated genes), results in specific cleavage of invading nucleic acid sequences recognized by the cell’s “memory” of past encounters. Here, we exploited the...

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Autores principales:	Alexander P. Hynes, Simon J. Labrie, Sylvain Moineau
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2016
Materias:	Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/e5ed4ae1150b4e7380f5f66b791592ca
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spelling	oai:doaj.org-article:e5ed4ae1150b4e7380f5f66b791592ca2021-11-15T15:50:17ZProgramming Native CRISPR Arrays for the Generation of Targeted Immunity10.1128/mBio.00202-162150-7511https://doaj.org/article/e5ed4ae1150b4e7380f5f66b791592ca2016-07-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mBio.00202-16https://doaj.org/toc/2150-7511ABSTRACT The adaptive immune system of prokaryotes, called CRISPR-Cas (clustered regularly interspaced short palindromic repeats and CRISPR-associated genes), results in specific cleavage of invading nucleic acid sequences recognized by the cell’s “memory” of past encounters. Here, we exploited the properties of native CRISPR-Cas systems to program the natural “memorization” process, efficiently generating immunity not only to a bacteriophage or plasmid but to any specifically chosen DNA sequence. IMPORTANCE CRISPR-Cas systems have entered the public consciousness as genome editing tools due to their readily programmable nature. In industrial settings, natural CRISPR-Cas immunity is already exploited to generate strains resistant to potentially disruptive viruses. However, the natural process by which bacteria acquire new target specificities (adaptation) is difficult to study and manipulate. The target against which immunity is conferred is selected stochastically. By biasing the immunization process, we offer a means to generate customized immunity, as well as provide a new tool to study adaptation.Alexander P. HynesSimon J. LabrieSylvain MoineauAmerican Society for MicrobiologyarticleMicrobiologyQR1-502ENmBio, Vol 7, Iss 3 (2016)
institution	DOAJ
collection	DOAJ
language	EN
topic	Microbiology QR1-502
spellingShingle	Microbiology QR1-502 Alexander P. Hynes Simon J. Labrie Sylvain Moineau Programming Native CRISPR Arrays for the Generation of Targeted Immunity
description	ABSTRACT The adaptive immune system of prokaryotes, called CRISPR-Cas (clustered regularly interspaced short palindromic repeats and CRISPR-associated genes), results in specific cleavage of invading nucleic acid sequences recognized by the cell’s “memory” of past encounters. Here, we exploited the properties of native CRISPR-Cas systems to program the natural “memorization” process, efficiently generating immunity not only to a bacteriophage or plasmid but to any specifically chosen DNA sequence. IMPORTANCE CRISPR-Cas systems have entered the public consciousness as genome editing tools due to their readily programmable nature. In industrial settings, natural CRISPR-Cas immunity is already exploited to generate strains resistant to potentially disruptive viruses. However, the natural process by which bacteria acquire new target specificities (adaptation) is difficult to study and manipulate. The target against which immunity is conferred is selected stochastically. By biasing the immunization process, we offer a means to generate customized immunity, as well as provide a new tool to study adaptation.
format	article
author	Alexander P. Hynes Simon J. Labrie Sylvain Moineau
author_facet	Alexander P. Hynes Simon J. Labrie Sylvain Moineau
author_sort	Alexander P. Hynes
title	Programming Native CRISPR Arrays for the Generation of Targeted Immunity
title_short	Programming Native CRISPR Arrays for the Generation of Targeted Immunity
title_full	Programming Native CRISPR Arrays for the Generation of Targeted Immunity
title_fullStr	Programming Native CRISPR Arrays for the Generation of Targeted Immunity
title_full_unstemmed	Programming Native CRISPR Arrays for the Generation of Targeted Immunity
title_sort	programming native crispr arrays for the generation of targeted immunity
publisher	American Society for Microbiology
publishDate	2016
url	https://doaj.org/article/e5ed4ae1150b4e7380f5f66b791592ca
work_keys_str_mv	AT alexanderphynes programmingnativecrisprarraysforthegenerationoftargetedimmunity AT simonjlabrie programmingnativecrisprarraysforthegenerationoftargetedimmunity AT sylvainmoineau programmingnativecrisprarraysforthegenerationoftargetedimmunity
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Programming Native CRISPR Arrays for the Generation of Targeted Immunity

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