Synergistic stabilization of a double mutant in chymotrypsin inhibitor 2 from a library screen in E. coli

Louise Hamborg et al. use a cell-based screen in E. coli to look for mutants of chymotrypsin inhibitor 2 which are either destabilized or stabilized, in an effort to identify a larger library of mutants which might improve stability prediction. Their work demonstrates how subtle conformational effec...

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Autores principales: Louise Hamborg, Daniele Granata, Johan G. Olsen, Jennifer Virginia Roche, Lasse Ebdrup Pedersen, Alex Toftgaard Nielsen, Kresten Lindorff-Larsen, Kaare Teilum
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2021
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Acceso en línea:https://doaj.org/article/e62f07713f7946109276026a00012c4c
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