Synergistic stabilization of a double mutant in chymotrypsin inhibitor 2 from a library screen in E. coli

Louise Hamborg et al. use a cell-based screen in E. coli to look for mutants of chymotrypsin inhibitor 2 which are either destabilized or stabilized, in an effort to identify a larger library of mutants which might improve stability prediction. Their work demonstrates how subtle conformational effec...

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Autores principales:	Louise Hamborg, Daniele Granata, Johan G. Olsen, Jennifer Virginia Roche, Lasse Ebdrup Pedersen, Alex Toftgaard Nielsen, Kresten Lindorff-Larsen, Kaare Teilum
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/e62f07713f7946109276026a00012c4c
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spelling	oai:doaj.org-article:e62f07713f7946109276026a00012c4c2021-12-02T17:08:42ZSynergistic stabilization of a double mutant in chymotrypsin inhibitor 2 from a library screen in E. coli10.1038/s42003-021-02490-72399-3642https://doaj.org/article/e62f07713f7946109276026a00012c4c2021-08-01T00:00:00Zhttps://doi.org/10.1038/s42003-021-02490-7https://doaj.org/toc/2399-3642Louise Hamborg et al. use a cell-based screen in E. coli to look for mutants of chymotrypsin inhibitor 2 which are either destabilized or stabilized, in an effort to identify a larger library of mutants which might improve stability prediction. Their work demonstrates how subtle conformational effects in proteins may modulate protein stability.Louise HamborgDaniele GranataJohan G. OlsenJennifer Virginia RocheLasse Ebdrup PedersenAlex Toftgaard NielsenKresten Lindorff-LarsenKaare TeilumNature PortfolioarticleBiology (General)QH301-705.5ENCommunications Biology, Vol 4, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Biology (General) QH301-705.5
spellingShingle	Biology (General) QH301-705.5 Louise Hamborg Daniele Granata Johan G. Olsen Jennifer Virginia Roche Lasse Ebdrup Pedersen Alex Toftgaard Nielsen Kresten Lindorff-Larsen Kaare Teilum Synergistic stabilization of a double mutant in chymotrypsin inhibitor 2 from a library screen in E. coli
description	Louise Hamborg et al. use a cell-based screen in E. coli to look for mutants of chymotrypsin inhibitor 2 which are either destabilized or stabilized, in an effort to identify a larger library of mutants which might improve stability prediction. Their work demonstrates how subtle conformational effects in proteins may modulate protein stability.
format	article
author	Louise Hamborg Daniele Granata Johan G. Olsen Jennifer Virginia Roche Lasse Ebdrup Pedersen Alex Toftgaard Nielsen Kresten Lindorff-Larsen Kaare Teilum
author_facet	Louise Hamborg Daniele Granata Johan G. Olsen Jennifer Virginia Roche Lasse Ebdrup Pedersen Alex Toftgaard Nielsen Kresten Lindorff-Larsen Kaare Teilum
author_sort	Louise Hamborg
title	Synergistic stabilization of a double mutant in chymotrypsin inhibitor 2 from a library screen in E. coli
title_short	Synergistic stabilization of a double mutant in chymotrypsin inhibitor 2 from a library screen in E. coli
title_full	Synergistic stabilization of a double mutant in chymotrypsin inhibitor 2 from a library screen in E. coli
title_fullStr	Synergistic stabilization of a double mutant in chymotrypsin inhibitor 2 from a library screen in E. coli
title_full_unstemmed	Synergistic stabilization of a double mutant in chymotrypsin inhibitor 2 from a library screen in E. coli
title_sort	synergistic stabilization of a double mutant in chymotrypsin inhibitor 2 from a library screen in e. coli
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/e62f07713f7946109276026a00012c4c
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Synergistic stabilization of a double mutant in chymotrypsin inhibitor 2 from a library screen in E. coli

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