Mechanical strain regulates osteoblast proliferation through integrin-mediated ERK activation.

Mechanical strain plays a critical role in the proliferation, differentiation and maturation of bone cells. As mechanical receptor cells, osteoblasts perceive and respond to stress force, such as those associated with compression, strain and shear stress. However, the underlying molecular mechanisms...

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Autores principales:	Yu-xian Yan, Yuan-wei Gong, Yong Guo, Qi Lv, Chun Guo, Yan Zhuang, Yuan Zhang, Ruixin Li, Xi-zheng Zhang
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/e6bb730e82ea44008eeda1bfb565b212
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spelling	oai:doaj.org-article:e6bb730e82ea44008eeda1bfb565b2122021-11-18T07:21:14ZMechanical strain regulates osteoblast proliferation through integrin-mediated ERK activation.1932-620310.1371/journal.pone.0035709https://doaj.org/article/e6bb730e82ea44008eeda1bfb565b2122012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22539993/?tool=EBIhttps://doaj.org/toc/1932-6203Mechanical strain plays a critical role in the proliferation, differentiation and maturation of bone cells. As mechanical receptor cells, osteoblasts perceive and respond to stress force, such as those associated with compression, strain and shear stress. However, the underlying molecular mechanisms of this process remain unclear. Using a four-point bending device, mouse MC3T3-E1 cells was exposed to mechanical tensile strain. Cell proliferation was determined to be most efficient when stimulated once a day by mechanical strain at a frequency of 0.5 Hz and intensities of 2500 µε with once a day, and a periodicity of 1 h/day for 3 days. The applied mechanical strain resulted in the altered expression of 1992 genes, 41 of which are involved in the mitogen-activated protein kinase (MAPK) signaling pathway. Activation of ERK by mechanical strain promoted cell proliferation and inactivation of ERK by PD98059 suppressed proliferation, confirming that ERK plays an important role in the response to mechanical strain. Furthermore, the membrane-associated receptors integrin β1 and integrin β5 were determined to regulate ERK activity and the proliferation of mechanical strain-treated MC3T3-E1 cells in opposite ways. The knockdown of integrin β1 led to the inhibition of ERK activity and cell proliferation, whereas the knockdown of integrin β5 led to the enhancement of both processes. This study proposes a novel mechanism by which mechanical strain regulates bone growth and remodeling.Yu-xian YanYuan-wei GongYong GuoQi LvChun GuoYan ZhuangYuan ZhangRuixin LiXi-zheng ZhangPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 4, p e35709 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yu-xian Yan Yuan-wei Gong Yong Guo Qi Lv Chun Guo Yan Zhuang Yuan Zhang Ruixin Li Xi-zheng Zhang Mechanical strain regulates osteoblast proliferation through integrin-mediated ERK activation.
description	Mechanical strain plays a critical role in the proliferation, differentiation and maturation of bone cells. As mechanical receptor cells, osteoblasts perceive and respond to stress force, such as those associated with compression, strain and shear stress. However, the underlying molecular mechanisms of this process remain unclear. Using a four-point bending device, mouse MC3T3-E1 cells was exposed to mechanical tensile strain. Cell proliferation was determined to be most efficient when stimulated once a day by mechanical strain at a frequency of 0.5 Hz and intensities of 2500 µε with once a day, and a periodicity of 1 h/day for 3 days. The applied mechanical strain resulted in the altered expression of 1992 genes, 41 of which are involved in the mitogen-activated protein kinase (MAPK) signaling pathway. Activation of ERK by mechanical strain promoted cell proliferation and inactivation of ERK by PD98059 suppressed proliferation, confirming that ERK plays an important role in the response to mechanical strain. Furthermore, the membrane-associated receptors integrin β1 and integrin β5 were determined to regulate ERK activity and the proliferation of mechanical strain-treated MC3T3-E1 cells in opposite ways. The knockdown of integrin β1 led to the inhibition of ERK activity and cell proliferation, whereas the knockdown of integrin β5 led to the enhancement of both processes. This study proposes a novel mechanism by which mechanical strain regulates bone growth and remodeling.
format	article
author	Yu-xian Yan Yuan-wei Gong Yong Guo Qi Lv Chun Guo Yan Zhuang Yuan Zhang Ruixin Li Xi-zheng Zhang
author_facet	Yu-xian Yan Yuan-wei Gong Yong Guo Qi Lv Chun Guo Yan Zhuang Yuan Zhang Ruixin Li Xi-zheng Zhang
author_sort	Yu-xian Yan
title	Mechanical strain regulates osteoblast proliferation through integrin-mediated ERK activation.
title_short	Mechanical strain regulates osteoblast proliferation through integrin-mediated ERK activation.
title_full	Mechanical strain regulates osteoblast proliferation through integrin-mediated ERK activation.
title_fullStr	Mechanical strain regulates osteoblast proliferation through integrin-mediated ERK activation.
title_full_unstemmed	Mechanical strain regulates osteoblast proliferation through integrin-mediated ERK activation.
title_sort	mechanical strain regulates osteoblast proliferation through integrin-mediated erk activation.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/e6bb730e82ea44008eeda1bfb565b212
work_keys_str_mv	AT yuxianyan mechanicalstrainregulatesosteoblastproliferationthroughintegrinmediatederkactivation AT yuanweigong mechanicalstrainregulatesosteoblastproliferationthroughintegrinmediatederkactivation AT yongguo mechanicalstrainregulatesosteoblastproliferationthroughintegrinmediatederkactivation AT qilv mechanicalstrainregulatesosteoblastproliferationthroughintegrinmediatederkactivation AT chunguo mechanicalstrainregulatesosteoblastproliferationthroughintegrinmediatederkactivation AT yanzhuang mechanicalstrainregulatesosteoblastproliferationthroughintegrinmediatederkactivation AT yuanzhang mechanicalstrainregulatesosteoblastproliferationthroughintegrinmediatederkactivation AT ruixinli mechanicalstrainregulatesosteoblastproliferationthroughintegrinmediatederkactivation AT xizhengzhang mechanicalstrainregulatesosteoblastproliferationthroughintegrinmediatederkactivation
_version_	1718423634655051776

Mechanical strain regulates osteoblast proliferation through integrin-mediated ERK activation.

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