OPTIMIZATION OF NEUROTRANSMITTERS SEPARATION UNDER HILIC CONDITIONS
This paper presents the optimization of a hydrophilic interaction chromatographic method for the separation of eleven catecholamines, indolamines and their precursors and metabolites. The studied parameters are: the organic modifier nature and percentage, the salt nature and concentration, the mobi...
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| Main Authors: | , |
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| Format: | article |
| Language: | EN |
| Published: |
Alma Mater Publishing House "Vasile Alecsandri" University of Bacau
2017
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| Subjects: | |
| Online Access: | https://doaj.org/article/e6ea3a7f651b4741a9eba2590ae81fc5 |
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| Summary: | This paper presents the optimization of a hydrophilic interaction chromatographic method for the separation of eleven catecholamines, indolamines and their precursors and metabolites. The studied parameters are: the organic modifier nature and percentage, the salt nature and concentration, the mobile phase pH and the column temperature. The best results in terms of separation were obtained using a mobile phase composed of acetonitrile and ammonium formate (150 mM pH 3) 85:15 v:v.
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