Anti-ganglioside Antibodies in Guillain-Barre Syndrome: A Novel Immunoblotting-Panel Assay

Objective: This study aimed to determine the diagnostic efficiency of a novel immunoblotting detection assay for anti-ganglioside antibodies (AGAs) in the Guillain–Barre syndrome (GBS).Method: Serum immunoglobulin (IgG and IgM) of AGAs were measured in 121 participants from a registered cohort study...

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Autores principales:	Jiting Zhu, Yuanyuan Zhang, Runyun Li, Yi Lin, Ying Fu, Yaping Yan, Wenli Zhu, Ning Wang, Zaiqiang Zhang, Guorong Xu
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	Guillain-Barre syndrome immunoblotting detection ganglioside antibodies peripheral neuropathy Neurology. Diseases of the nervous system RC346-429
Acceso en línea:	https://doaj.org/article/e72bb74dd7594ec39eb00b7a05b7de58
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spelling	oai:doaj.org-article:e72bb74dd7594ec39eb00b7a05b7de582021-11-30T21:21:12ZAnti-ganglioside Antibodies in Guillain-Barre Syndrome: A Novel Immunoblotting-Panel Assay1664-229510.3389/fneur.2021.760889https://doaj.org/article/e72bb74dd7594ec39eb00b7a05b7de582021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fneur.2021.760889/fullhttps://doaj.org/toc/1664-2295Objective: This study aimed to determine the diagnostic efficiency of a novel immunoblotting detection assay for anti-ganglioside antibodies (AGAs) in the Guillain–Barre syndrome (GBS).Method: Serum immunoglobulin (IgG and IgM) of AGAs were measured in 121 participants from a registered cohort study of immune-mediated neuropathies and 29 healthy controls by immunoblotting panel assay. Sensitivity, specificity, and positive predictive value (PPV) of the assay were compared to calculate the diagnostic accuracy.Result: In our cohort, any of the AGAs were positive in 42.4% of the GBS patients. The sensitivity and specificity of AGAs (both IgG and IgM) in the diagnosis of GSB were 42 and 76% while for IgG-AGAs were 35 and 87%. AGAs positivity had a significant association with the AMAN subtype (P = 0.0004), and the sensitivity, specificity of AGAs in AMAN were 86, 69%, respectively with high (AUC = 0.78, p = 0.002) discriminative powers. GM1-IgG AGA was more common and specific to AMAN patients than other GBS forms (p = 0.008).Conclusion: Our novel immunoblotting detection assay could complement GBS diagnosis. IgG-AGAs were more likely to be detected in GBS, and GM1-IgG AGA could assist AMAN diagnosis.Jiting ZhuYuanyuan ZhangRunyun LiYi LinYing FuYaping YanWenli ZhuNing WangZaiqiang ZhangGuorong XuFrontiers Media S.A.articleGuillain-Barre syndromeimmunoblotting detectiongangliosideantibodiesperipheral neuropathyNeurology. Diseases of the nervous systemRC346-429ENFrontiers in Neurology, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Guillain-Barre syndrome immunoblotting detection ganglioside antibodies peripheral neuropathy Neurology. Diseases of the nervous system RC346-429
spellingShingle	Guillain-Barre syndrome immunoblotting detection ganglioside antibodies peripheral neuropathy Neurology. Diseases of the nervous system RC346-429 Jiting Zhu Yuanyuan Zhang Runyun Li Yi Lin Ying Fu Yaping Yan Wenli Zhu Ning Wang Zaiqiang Zhang Guorong Xu Anti-ganglioside Antibodies in Guillain-Barre Syndrome: A Novel Immunoblotting-Panel Assay
description	Objective: This study aimed to determine the diagnostic efficiency of a novel immunoblotting detection assay for anti-ganglioside antibodies (AGAs) in the Guillain–Barre syndrome (GBS).Method: Serum immunoglobulin (IgG and IgM) of AGAs were measured in 121 participants from a registered cohort study of immune-mediated neuropathies and 29 healthy controls by immunoblotting panel assay. Sensitivity, specificity, and positive predictive value (PPV) of the assay were compared to calculate the diagnostic accuracy.Result: In our cohort, any of the AGAs were positive in 42.4% of the GBS patients. The sensitivity and specificity of AGAs (both IgG and IgM) in the diagnosis of GSB were 42 and 76% while for IgG-AGAs were 35 and 87%. AGAs positivity had a significant association with the AMAN subtype (P = 0.0004), and the sensitivity, specificity of AGAs in AMAN were 86, 69%, respectively with high (AUC = 0.78, p = 0.002) discriminative powers. GM1-IgG AGA was more common and specific to AMAN patients than other GBS forms (p = 0.008).Conclusion: Our novel immunoblotting detection assay could complement GBS diagnosis. IgG-AGAs were more likely to be detected in GBS, and GM1-IgG AGA could assist AMAN diagnosis.
format	article
author	Jiting Zhu Yuanyuan Zhang Runyun Li Yi Lin Ying Fu Yaping Yan Wenli Zhu Ning Wang Zaiqiang Zhang Guorong Xu
author_facet	Jiting Zhu Yuanyuan Zhang Runyun Li Yi Lin Ying Fu Yaping Yan Wenli Zhu Ning Wang Zaiqiang Zhang Guorong Xu
author_sort	Jiting Zhu
title	Anti-ganglioside Antibodies in Guillain-Barre Syndrome: A Novel Immunoblotting-Panel Assay
title_short	Anti-ganglioside Antibodies in Guillain-Barre Syndrome: A Novel Immunoblotting-Panel Assay
title_full	Anti-ganglioside Antibodies in Guillain-Barre Syndrome: A Novel Immunoblotting-Panel Assay
title_fullStr	Anti-ganglioside Antibodies in Guillain-Barre Syndrome: A Novel Immunoblotting-Panel Assay
title_full_unstemmed	Anti-ganglioside Antibodies in Guillain-Barre Syndrome: A Novel Immunoblotting-Panel Assay
title_sort	anti-ganglioside antibodies in guillain-barre syndrome: a novel immunoblotting-panel assay
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/e72bb74dd7594ec39eb00b7a05b7de58
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Anti-ganglioside Antibodies in Guillain-Barre Syndrome: A Novel Immunoblotting-Panel Assay

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