A Pharmacokinetic Study of Ephedrine and Pseudoephedrine after Oral Administration of Ojeok-San by Validated LC-MS/MS Method in Human Plasma
A sensitive and reproducible liquid chromatography-tandem mass spectrometry (LC-MS/MS) system was developed and fully validated for the simultaneous determination of ephedrine and pseudoephedrine in human plasma after oral administration of the herbal prescription Ojeok-san (OJS); 2-phenylethylamine...
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2021
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oai:doaj.org-article:e90ab1bdd10343f495d7e182cba628782021-11-25T18:28:58ZA Pharmacokinetic Study of Ephedrine and Pseudoephedrine after Oral Administration of Ojeok-San by Validated LC-MS/MS Method in Human Plasma10.3390/molecules262269911420-3049https://doaj.org/article/e90ab1bdd10343f495d7e182cba628782021-11-01T00:00:00Zhttps://www.mdpi.com/1420-3049/26/22/6991https://doaj.org/toc/1420-3049A sensitive and reproducible liquid chromatography-tandem mass spectrometry (LC-MS/MS) system was developed and fully validated for the simultaneous determination of ephedrine and pseudoephedrine in human plasma after oral administration of the herbal prescription Ojeok-san (OJS); 2-phenylethylamine was used as the internal standard (IS). Both compounds presented a linear calibration curve (<i>r</i><sup>2</sup> ≥ 0.99) over a concentration range of 0.2–50 ng/mL. The developed method was fully validated in terms of selectivity, lower limit of quantitation, precision, accuracy, recovery, matrix effect, and stability, according to the regulatory guidelines from the U.S. Food and Drug Administration and the Korea Ministry of Food and Drug Safety. This validated method was successfully applied for the pharmacokinetic assessment of ephedrine and pseudoephedrine in 20 healthy Korean volunteers administered OJS.Sooyoung LeeWang-Seob ShimHeejo YooSanghee ChoiJiyoung YoonKwang-Young LeeEun-Kyoung ChungByung-Cheol LeeSung-Vin YimBo-Hyung KimKyung-Tae LeeMDPI AGarticleephedrinepseudoephedrineliquid chromatography-tandem mass spectrometrybioanalytical method validationpharmacokineticshealthy volunteersOrganic chemistryQD241-441ENMolecules, Vol 26, Iss 6991, p 6991 (2021) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
ephedrine pseudoephedrine liquid chromatography-tandem mass spectrometry bioanalytical method validation pharmacokinetics healthy volunteers Organic chemistry QD241-441 |
| spellingShingle |
ephedrine pseudoephedrine liquid chromatography-tandem mass spectrometry bioanalytical method validation pharmacokinetics healthy volunteers Organic chemistry QD241-441 Sooyoung Lee Wang-Seob Shim Heejo Yoo Sanghee Choi Jiyoung Yoon Kwang-Young Lee Eun-Kyoung Chung Byung-Cheol Lee Sung-Vin Yim Bo-Hyung Kim Kyung-Tae Lee A Pharmacokinetic Study of Ephedrine and Pseudoephedrine after Oral Administration of Ojeok-San by Validated LC-MS/MS Method in Human Plasma |
| description |
A sensitive and reproducible liquid chromatography-tandem mass spectrometry (LC-MS/MS) system was developed and fully validated for the simultaneous determination of ephedrine and pseudoephedrine in human plasma after oral administration of the herbal prescription Ojeok-san (OJS); 2-phenylethylamine was used as the internal standard (IS). Both compounds presented a linear calibration curve (<i>r</i><sup>2</sup> ≥ 0.99) over a concentration range of 0.2–50 ng/mL. The developed method was fully validated in terms of selectivity, lower limit of quantitation, precision, accuracy, recovery, matrix effect, and stability, according to the regulatory guidelines from the U.S. Food and Drug Administration and the Korea Ministry of Food and Drug Safety. This validated method was successfully applied for the pharmacokinetic assessment of ephedrine and pseudoephedrine in 20 healthy Korean volunteers administered OJS. |
| format |
article |
| author |
Sooyoung Lee Wang-Seob Shim Heejo Yoo Sanghee Choi Jiyoung Yoon Kwang-Young Lee Eun-Kyoung Chung Byung-Cheol Lee Sung-Vin Yim Bo-Hyung Kim Kyung-Tae Lee |
| author_facet |
Sooyoung Lee Wang-Seob Shim Heejo Yoo Sanghee Choi Jiyoung Yoon Kwang-Young Lee Eun-Kyoung Chung Byung-Cheol Lee Sung-Vin Yim Bo-Hyung Kim Kyung-Tae Lee |
| author_sort |
Sooyoung Lee |
| title |
A Pharmacokinetic Study of Ephedrine and Pseudoephedrine after Oral Administration of Ojeok-San by Validated LC-MS/MS Method in Human Plasma |
| title_short |
A Pharmacokinetic Study of Ephedrine and Pseudoephedrine after Oral Administration of Ojeok-San by Validated LC-MS/MS Method in Human Plasma |
| title_full |
A Pharmacokinetic Study of Ephedrine and Pseudoephedrine after Oral Administration of Ojeok-San by Validated LC-MS/MS Method in Human Plasma |
| title_fullStr |
A Pharmacokinetic Study of Ephedrine and Pseudoephedrine after Oral Administration of Ojeok-San by Validated LC-MS/MS Method in Human Plasma |
| title_full_unstemmed |
A Pharmacokinetic Study of Ephedrine and Pseudoephedrine after Oral Administration of Ojeok-San by Validated LC-MS/MS Method in Human Plasma |
| title_sort |
pharmacokinetic study of ephedrine and pseudoephedrine after oral administration of ojeok-san by validated lc-ms/ms method in human plasma |
| publisher |
MDPI AG |
| publishDate |
2021 |
| url |
https://doaj.org/article/e90ab1bdd10343f495d7e182cba62878 |
| work_keys_str_mv |
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