Real-time relative qPCR without reference to control samples and estimation of run-specific PCR parameters from run-internal mini-standard curves.

<h4>Background</h4>Real-Time quantitative PCR is an important tool in research and clinical settings. Here, we describe two new approaches that broaden the scope of real-time quantitative PCR; namely, run-internal mini standard curves (RIMS) and direct real-time relative quantitative PCR...

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Autores principales: Jens Magnus Bernth Jensen, Mikkel Steen Petersen, Marc Stegger, Lars J Østergaard, Bjarne K Møller
Formato: article
Lenguaje:EN
Publicado: Public Library of Science (PLoS) 2010
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Q
Acceso en línea:https://doaj.org/article/e91401e5d8564dbb95be9489ab8cde4f
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