Real-time relative qPCR without reference to control samples and estimation of run-specific PCR parameters from run-internal mini-standard curves.

<h4>Background</h4>Real-Time quantitative PCR is an important tool in research and clinical settings. Here, we describe two new approaches that broaden the scope of real-time quantitative PCR; namely, run-internal mini standard curves (RIMS) and direct real-time relative quantitative PCR...

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Bibliographic Details
Main Authors: Jens Magnus Bernth Jensen, Mikkel Steen Petersen, Marc Stegger, Lars J Østergaard, Bjarne K Møller
Format: article
Language:EN
Published: Public Library of Science (PLoS) 2010
Subjects:
R
Q
Online Access:https://doaj.org/article/e91401e5d8564dbb95be9489ab8cde4f
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