Optimization and characterization of enterocin Enterococcus faecalis K2B1 isolated from Toraja’s Belang Buffalo Milk, South Sulawesi, Indonesia

Abstract. Alang H, Kusnadi J, Ardyati T, Suharjono. 2020. Optimization and characterization of enterocin Enterococcus faecalis K2B1 isolated from Toraja’s Belang Buffalo Milk, South Sulawesi, Indonesia. Biodiversitas 21: 1236-1242. The growing bacterial resistance needs to be controlled with effecti...

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Autores principales:	Hasria Alang, JONI KUSNADI, TRI ARDYATI, SUHARJONO
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2020
Materias:	blis, characterization, enterocin, enterococcus faecalis, purification Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/e9424a0b11d744718dc0013d61e11a27
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spelling	oai:doaj.org-article:e9424a0b11d744718dc0013d61e11a272021-11-22T00:28:55ZOptimization and characterization of enterocin Enterococcus faecalis K2B1 isolated from Toraja’s Belang Buffalo Milk, South Sulawesi, Indonesia1412-033X2085-472210.13057/biodiv/d210351https://doaj.org/article/e9424a0b11d744718dc0013d61e11a272020-02-01T00:00:00Zhttps://smujo.id/biodiv/article/view/5016https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Abstract. Alang H, Kusnadi J, Ardyati T, Suharjono. 2020. Optimization and characterization of enterocin Enterococcus faecalis K2B1 isolated from Toraja’s Belang Buffalo Milk, South Sulawesi, Indonesia. Biodiversitas 21: 1236-1242. The growing bacterial resistance needs to be controlled with effective antimicrobials. Bacteriocins are proteinaceous toxins produced by bacteria to inhibit the growth of pathogens. Bacteriocin industry has substantially grown, replacing the role of chemical preservatives in enhancing shelf-life and food safety. Bacteriocin produced by genera Enterococcus can be used as an antimicrobial against pathogen. In this study, we used Enterococcus faecalis K2B1 which was first isolated from Toraja’s Belang Buffalo Milk Makassar, Indonesia. The study aimed to optimization and characterization of Enterocinroduced by Enterococcus faecalis K2B1, isolated from Toraja’s Belang buffalo milk. Research Method: including optimization and characterization of antimicrobial metabolite (BLIS) using MRS broth with different initial pH (pH 6, 7 and 8), and partial purification with ammonium sulfate on different concentration (40, 60 and 80 %). Result showed that BLIS production was most optimal on MRS broth at initial pH of 8 after13th hour fermentation, at late exponential phase. BLIS characterization visibly active at high temperatures and very wide range of pH, and disappeared after treating with proteinase-K. Crude had a broad spectrum, was sensitive to Proteinase-K and estimated to have molecular weight of 5 kDa.Hasria AlangJONI KUSNADITRI ARDYATISUHARJONOMBI & UNS Soloarticleblis, characterization, enterocin, enterococcus faecalis, purificationBiology (General)QH301-705.5ENBiodiversitas, Vol 21, Iss 3 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	blis, characterization, enterocin, enterococcus faecalis, purification Biology (General) QH301-705.5
spellingShingle	blis, characterization, enterocin, enterococcus faecalis, purification Biology (General) QH301-705.5 Hasria Alang JONI KUSNADI TRI ARDYATI SUHARJONO Optimization and characterization of enterocin Enterococcus faecalis K2B1 isolated from Toraja’s Belang Buffalo Milk, South Sulawesi, Indonesia
description	Abstract. Alang H, Kusnadi J, Ardyati T, Suharjono. 2020. Optimization and characterization of enterocin Enterococcus faecalis K2B1 isolated from Toraja’s Belang Buffalo Milk, South Sulawesi, Indonesia. Biodiversitas 21: 1236-1242. The growing bacterial resistance needs to be controlled with effective antimicrobials. Bacteriocins are proteinaceous toxins produced by bacteria to inhibit the growth of pathogens. Bacteriocin industry has substantially grown, replacing the role of chemical preservatives in enhancing shelf-life and food safety. Bacteriocin produced by genera Enterococcus can be used as an antimicrobial against pathogen. In this study, we used Enterococcus faecalis K2B1 which was first isolated from Toraja’s Belang Buffalo Milk Makassar, Indonesia. The study aimed to optimization and characterization of Enterocinroduced by Enterococcus faecalis K2B1, isolated from Toraja’s Belang buffalo milk. Research Method: including optimization and characterization of antimicrobial metabolite (BLIS) using MRS broth with different initial pH (pH 6, 7 and 8), and partial purification with ammonium sulfate on different concentration (40, 60 and 80 %). Result showed that BLIS production was most optimal on MRS broth at initial pH of 8 after13th hour fermentation, at late exponential phase. BLIS characterization visibly active at high temperatures and very wide range of pH, and disappeared after treating with proteinase-K. Crude had a broad spectrum, was sensitive to Proteinase-K and estimated to have molecular weight of 5 kDa.
format	article
author	Hasria Alang JONI KUSNADI TRI ARDYATI SUHARJONO
author_facet	Hasria Alang JONI KUSNADI TRI ARDYATI SUHARJONO
author_sort	Hasria Alang
title	Optimization and characterization of enterocin Enterococcus faecalis K2B1 isolated from Toraja’s Belang Buffalo Milk, South Sulawesi, Indonesia
title_short	Optimization and characterization of enterocin Enterococcus faecalis K2B1 isolated from Toraja’s Belang Buffalo Milk, South Sulawesi, Indonesia
title_full	Optimization and characterization of enterocin Enterococcus faecalis K2B1 isolated from Toraja’s Belang Buffalo Milk, South Sulawesi, Indonesia
title_fullStr	Optimization and characterization of enterocin Enterococcus faecalis K2B1 isolated from Toraja’s Belang Buffalo Milk, South Sulawesi, Indonesia
title_full_unstemmed	Optimization and characterization of enterocin Enterococcus faecalis K2B1 isolated from Toraja’s Belang Buffalo Milk, South Sulawesi, Indonesia
title_sort	optimization and characterization of enterocin enterococcus faecalis k2b1 isolated from toraja’s belang buffalo milk, south sulawesi, indonesia
publisher	MBI & UNS Solo
publishDate	2020
url	https://doaj.org/article/e9424a0b11d744718dc0013d61e11a27
work_keys_str_mv	AT hasriaalang optimizationandcharacterizationofenterocinenterococcusfaecalisk2b1isolatedfromtorajasbelangbuffalomilksouthsulawesiindonesia AT jonikusnadi optimizationandcharacterizationofenterocinenterococcusfaecalisk2b1isolatedfromtorajasbelangbuffalomilksouthsulawesiindonesia AT triardyati optimizationandcharacterizationofenterocinenterococcusfaecalisk2b1isolatedfromtorajasbelangbuffalomilksouthsulawesiindonesia AT suharjono optimizationandcharacterizationofenterocinenterococcusfaecalisk2b1isolatedfromtorajasbelangbuffalomilksouthsulawesiindonesia
_version_	1718418615948017664

Optimization and characterization of enterocin Enterococcus faecalis K2B1 isolated from Toraja’s Belang Buffalo Milk, South Sulawesi, Indonesia

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