The auxin-inducible degron 2 technology provides sharp degradation control in yeast, mammalian cells, and mice

Auxin-inducible degron systems can be leaky and require high doses of auxin. Here the authors establish AID2 which uses an OsTIR1 mutant and the ligand 5-Ph-IAA to overcome these problems and establish AID-mediated target depletion in mice.

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Autores principales:	Aisha Yesbolatova, Yuichiro Saito, Naomi Kitamoto, Hatsune Makino-Itou, Rieko Ajima, Risako Nakano, Hirofumi Nakaoka, Kosuke Fukui, Kanae Gamo, Yusuke Tominari, Haruki Takeuchi, Yumiko Saga, Ken-ichiro Hayashi, Masato T. Kanemaki
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/e96ad288aa7749f7bdb3f1e9cc207dea
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spelling	oai:doaj.org-article:e96ad288aa7749f7bdb3f1e9cc207dea2021-12-02T17:31:11ZThe auxin-inducible degron 2 technology provides sharp degradation control in yeast, mammalian cells, and mice10.1038/s41467-020-19532-z2041-1723https://doaj.org/article/e96ad288aa7749f7bdb3f1e9cc207dea2020-11-01T00:00:00Zhttps://doi.org/10.1038/s41467-020-19532-zhttps://doaj.org/toc/2041-1723Auxin-inducible degron systems can be leaky and require high doses of auxin. Here the authors establish AID2 which uses an OsTIR1 mutant and the ligand 5-Ph-IAA to overcome these problems and establish AID-mediated target depletion in mice.Aisha YesbolatovaYuichiro SaitoNaomi KitamotoHatsune Makino-ItouRieko AjimaRisako NakanoHirofumi NakaokaKosuke FukuiKanae GamoYusuke TominariHaruki TakeuchiYumiko SagaKen-ichiro HayashiMasato T. KanemakiNature PortfolioarticleScienceQENNature Communications, Vol 11, Iss 1, Pp 1-13 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Aisha Yesbolatova Yuichiro Saito Naomi Kitamoto Hatsune Makino-Itou Rieko Ajima Risako Nakano Hirofumi Nakaoka Kosuke Fukui Kanae Gamo Yusuke Tominari Haruki Takeuchi Yumiko Saga Ken-ichiro Hayashi Masato T. Kanemaki The auxin-inducible degron 2 technology provides sharp degradation control in yeast, mammalian cells, and mice
description	Auxin-inducible degron systems can be leaky and require high doses of auxin. Here the authors establish AID2 which uses an OsTIR1 mutant and the ligand 5-Ph-IAA to overcome these problems and establish AID-mediated target depletion in mice.
format	article
author	Aisha Yesbolatova Yuichiro Saito Naomi Kitamoto Hatsune Makino-Itou Rieko Ajima Risako Nakano Hirofumi Nakaoka Kosuke Fukui Kanae Gamo Yusuke Tominari Haruki Takeuchi Yumiko Saga Ken-ichiro Hayashi Masato T. Kanemaki
author_facet	Aisha Yesbolatova Yuichiro Saito Naomi Kitamoto Hatsune Makino-Itou Rieko Ajima Risako Nakano Hirofumi Nakaoka Kosuke Fukui Kanae Gamo Yusuke Tominari Haruki Takeuchi Yumiko Saga Ken-ichiro Hayashi Masato T. Kanemaki
author_sort	Aisha Yesbolatova
title	The auxin-inducible degron 2 technology provides sharp degradation control in yeast, mammalian cells, and mice
title_short	The auxin-inducible degron 2 technology provides sharp degradation control in yeast, mammalian cells, and mice
title_full	The auxin-inducible degron 2 technology provides sharp degradation control in yeast, mammalian cells, and mice
title_fullStr	The auxin-inducible degron 2 technology provides sharp degradation control in yeast, mammalian cells, and mice
title_full_unstemmed	The auxin-inducible degron 2 technology provides sharp degradation control in yeast, mammalian cells, and mice
title_sort	auxin-inducible degron 2 technology provides sharp degradation control in yeast, mammalian cells, and mice
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/e96ad288aa7749f7bdb3f1e9cc207dea
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The auxin-inducible degron 2 technology provides sharp degradation control in yeast, mammalian cells, and mice

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