Reevaluation of ANS binding to human and bovine serum albumins: key role of equilibrium microdialysis in ligand - receptor binding characterization.
In this work we return to the problem of the determination of ligand-receptor binding stoichiometry and binding constants. In many cases the ligand is a fluorescent dye which has low fluorescence quantum yield in free state but forms highly fluorescent complex with target receptor. That is why many...
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Main Authors: | , , , |
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Format: | article |
Language: | EN |
Published: |
Public Library of Science (PLoS)
2012
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Subjects: | |
Online Access: | https://doaj.org/article/e99d26ee52f14569bed3092b9c3310b5 |
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