Fast preparation of RG-I enriched ultra-low molecular weight pectin by an ultrasound accelerated Fenton process

Abstract Pectin, a natural polysaccharide found in the cell wall of most higher plant such as citrus, has drawn much attention due to its potential beneficial role in facilitating the treatment of many diseases like cancer, hyper cholesterol and diabetes. However, the broad application of pectin fac...

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Autores principales:	Zijian Zhi, Jianle Chen, Shan Li, Wenjun Wang, Rui Huang, Donghong Liu, Tian Ding, Robert John Linhardt, Shiguo Chen, Xingqian Ye
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/eaab67833a7c4c82a41ecd15b187cf5e
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spelling	oai:doaj.org-article:eaab67833a7c4c82a41ecd15b187cf5e2021-12-02T11:41:20ZFast preparation of RG-I enriched ultra-low molecular weight pectin by an ultrasound accelerated Fenton process10.1038/s41598-017-00572-32045-2322https://doaj.org/article/eaab67833a7c4c82a41ecd15b187cf5e2017-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-00572-3https://doaj.org/toc/2045-2322Abstract Pectin, a natural polysaccharide found in the cell wall of most higher plant such as citrus, has drawn much attention due to its potential beneficial role in facilitating the treatment of many diseases like cancer, hyper cholesterol and diabetes. However, the broad application of pectin faces great limitations as the large molecular size of pectin severely prevents its bioavailability in vivo. In this study, we report an effective and highly convenient approach to degrade natural pectin into lower molecular pectin. By combining ultrasound with Fenton system (US-Fenton), we show that ultrasound synergistically enhances the efficiency of Fenton reaction to degrade pectin into 5.5 kDa within only 35 minutes. Importantly, RG-I domain, the most effective portion of natural pectin, was well preserved and highly enriched. In addition, the antioxidant activities of US-Fenton-treated pectin was significantly elevated. The mechanism of this novel observation was further investigated through the multiple structural analyses including HPLC, IR and NMR. Taken together, we present a novel and convenient approach to generate ultra-low molecular weight pectin with high efficiency and higher bioactivity. We expect our approach will have broader applications in improving the bioavailability and bioactivity of other polysaccharide-based natural compounds.Zijian ZhiJianle ChenShan LiWenjun WangRui HuangDonghong LiuTian DingRobert John LinhardtShiguo ChenXingqian YeNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Zijian Zhi Jianle Chen Shan Li Wenjun Wang Rui Huang Donghong Liu Tian Ding Robert John Linhardt Shiguo Chen Xingqian Ye Fast preparation of RG-I enriched ultra-low molecular weight pectin by an ultrasound accelerated Fenton process
description	Abstract Pectin, a natural polysaccharide found in the cell wall of most higher plant such as citrus, has drawn much attention due to its potential beneficial role in facilitating the treatment of many diseases like cancer, hyper cholesterol and diabetes. However, the broad application of pectin faces great limitations as the large molecular size of pectin severely prevents its bioavailability in vivo. In this study, we report an effective and highly convenient approach to degrade natural pectin into lower molecular pectin. By combining ultrasound with Fenton system (US-Fenton), we show that ultrasound synergistically enhances the efficiency of Fenton reaction to degrade pectin into 5.5 kDa within only 35 minutes. Importantly, RG-I domain, the most effective portion of natural pectin, was well preserved and highly enriched. In addition, the antioxidant activities of US-Fenton-treated pectin was significantly elevated. The mechanism of this novel observation was further investigated through the multiple structural analyses including HPLC, IR and NMR. Taken together, we present a novel and convenient approach to generate ultra-low molecular weight pectin with high efficiency and higher bioactivity. We expect our approach will have broader applications in improving the bioavailability and bioactivity of other polysaccharide-based natural compounds.
format	article
author	Zijian Zhi Jianle Chen Shan Li Wenjun Wang Rui Huang Donghong Liu Tian Ding Robert John Linhardt Shiguo Chen Xingqian Ye
author_facet	Zijian Zhi Jianle Chen Shan Li Wenjun Wang Rui Huang Donghong Liu Tian Ding Robert John Linhardt Shiguo Chen Xingqian Ye
author_sort	Zijian Zhi
title	Fast preparation of RG-I enriched ultra-low molecular weight pectin by an ultrasound accelerated Fenton process
title_short	Fast preparation of RG-I enriched ultra-low molecular weight pectin by an ultrasound accelerated Fenton process
title_full	Fast preparation of RG-I enriched ultra-low molecular weight pectin by an ultrasound accelerated Fenton process
title_fullStr	Fast preparation of RG-I enriched ultra-low molecular weight pectin by an ultrasound accelerated Fenton process
title_full_unstemmed	Fast preparation of RG-I enriched ultra-low molecular weight pectin by an ultrasound accelerated Fenton process
title_sort	fast preparation of rg-i enriched ultra-low molecular weight pectin by an ultrasound accelerated fenton process
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/eaab67833a7c4c82a41ecd15b187cf5e
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Fast preparation of RG-I enriched ultra-low molecular weight pectin by an ultrasound accelerated Fenton process

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