Generation of auxin inducible degron (AID) knock-in cell lines for targeted protein degradation in mammalian cells
Summary: Targeted protein degradation using degrons, such as the mini-Auxin-inducible degron (mAID), has an advantage over genetic silencing/knockout. However, the efficiency of sgRNA, homologous recombination, tedious expansion, and screening single clones makes the process of tagging endogenous pr...
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| Main Authors: | , , |
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| Format: | article |
| Language: | EN |
| Published: |
Elsevier
2021
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| Subjects: | |
| Online Access: | https://doaj.org/article/eabd39a47e3e4e508f16a6a2b0613cc0 |
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