Flat-top TIRF illumination boosts DNA-PAINT imaging and quantification

The use of TIRF microscopy for DNA-PAINT experiments is limited by inhomogeneous illumination. Here the authors show that quantitative analysis of single-molecule TIRF experiments can be improved by using a segment-wise analysis approach and overcome by using a beam-shaping device to give a flat-top...

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Autores principales: Florian Stehr, Johannes Stein, Florian Schueder, Petra Schwille, Ralf Jungmann
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2019
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Acceso en línea:https://doaj.org/article/ead3c03afa5a468fbe0ee0e7061d0a7a
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