Flat-top TIRF illumination boosts DNA-PAINT imaging and quantification
The use of TIRF microscopy for DNA-PAINT experiments is limited by inhomogeneous illumination. Here the authors show that quantitative analysis of single-molecule TIRF experiments can be improved by using a segment-wise analysis approach and overcome by using a beam-shaping device to give a flat-top...
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Nature Portfolio
2019
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oai:doaj.org-article:ead3c03afa5a468fbe0ee0e7061d0a7a2021-12-02T17:01:59ZFlat-top TIRF illumination boosts DNA-PAINT imaging and quantification10.1038/s41467-019-09064-62041-1723https://doaj.org/article/ead3c03afa5a468fbe0ee0e7061d0a7a2019-03-01T00:00:00Zhttps://doi.org/10.1038/s41467-019-09064-6https://doaj.org/toc/2041-1723The use of TIRF microscopy for DNA-PAINT experiments is limited by inhomogeneous illumination. Here the authors show that quantitative analysis of single-molecule TIRF experiments can be improved by using a segment-wise analysis approach and overcome by using a beam-shaping device to give a flat-top illumination profile.Florian StehrJohannes SteinFlorian SchuederPetra SchwilleRalf JungmannNature PortfolioarticleScienceQENNature Communications, Vol 10, Iss 1, Pp 1-8 (2019) |
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Science Q Florian Stehr Johannes Stein Florian Schueder Petra Schwille Ralf Jungmann Flat-top TIRF illumination boosts DNA-PAINT imaging and quantification |
description |
The use of TIRF microscopy for DNA-PAINT experiments is limited by inhomogeneous illumination. Here the authors show that quantitative analysis of single-molecule TIRF experiments can be improved by using a segment-wise analysis approach and overcome by using a beam-shaping device to give a flat-top illumination profile. |
format |
article |
author |
Florian Stehr Johannes Stein Florian Schueder Petra Schwille Ralf Jungmann |
author_facet |
Florian Stehr Johannes Stein Florian Schueder Petra Schwille Ralf Jungmann |
author_sort |
Florian Stehr |
title |
Flat-top TIRF illumination boosts DNA-PAINT imaging and quantification |
title_short |
Flat-top TIRF illumination boosts DNA-PAINT imaging and quantification |
title_full |
Flat-top TIRF illumination boosts DNA-PAINT imaging and quantification |
title_fullStr |
Flat-top TIRF illumination boosts DNA-PAINT imaging and quantification |
title_full_unstemmed |
Flat-top TIRF illumination boosts DNA-PAINT imaging and quantification |
title_sort |
flat-top tirf illumination boosts dna-paint imaging and quantification |
publisher |
Nature Portfolio |
publishDate |
2019 |
url |
https://doaj.org/article/ead3c03afa5a468fbe0ee0e7061d0a7a |
work_keys_str_mv |
AT florianstehr flattoptirfilluminationboostsdnapaintimagingandquantification AT johannesstein flattoptirfilluminationboostsdnapaintimagingandquantification AT florianschueder flattoptirfilluminationboostsdnapaintimagingandquantification AT petraschwille flattoptirfilluminationboostsdnapaintimagingandquantification AT ralfjungmann flattoptirfilluminationboostsdnapaintimagingandquantification |
_version_ |
1718382002599624704 |