Flat-top TIRF illumination boosts DNA-PAINT imaging and quantification

The use of TIRF microscopy for DNA-PAINT experiments is limited by inhomogeneous illumination. Here the authors show that quantitative analysis of single-molecule TIRF experiments can be improved by using a segment-wise analysis approach and overcome by using a beam-shaping device to give a flat-top...

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Autores principales: Florian Stehr, Johannes Stein, Florian Schueder, Petra Schwille, Ralf Jungmann
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2019
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Acceso en línea:https://doaj.org/article/ead3c03afa5a468fbe0ee0e7061d0a7a
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spelling oai:doaj.org-article:ead3c03afa5a468fbe0ee0e7061d0a7a2021-12-02T17:01:59ZFlat-top TIRF illumination boosts DNA-PAINT imaging and quantification10.1038/s41467-019-09064-62041-1723https://doaj.org/article/ead3c03afa5a468fbe0ee0e7061d0a7a2019-03-01T00:00:00Zhttps://doi.org/10.1038/s41467-019-09064-6https://doaj.org/toc/2041-1723The use of TIRF microscopy for DNA-PAINT experiments is limited by inhomogeneous illumination. Here the authors show that quantitative analysis of single-molecule TIRF experiments can be improved by using a segment-wise analysis approach and overcome by using a beam-shaping device to give a flat-top illumination profile.Florian StehrJohannes SteinFlorian SchuederPetra SchwilleRalf JungmannNature PortfolioarticleScienceQENNature Communications, Vol 10, Iss 1, Pp 1-8 (2019)
institution DOAJ
collection DOAJ
language EN
topic Science
Q
spellingShingle Science
Q
Florian Stehr
Johannes Stein
Florian Schueder
Petra Schwille
Ralf Jungmann
Flat-top TIRF illumination boosts DNA-PAINT imaging and quantification
description The use of TIRF microscopy for DNA-PAINT experiments is limited by inhomogeneous illumination. Here the authors show that quantitative analysis of single-molecule TIRF experiments can be improved by using a segment-wise analysis approach and overcome by using a beam-shaping device to give a flat-top illumination profile.
format article
author Florian Stehr
Johannes Stein
Florian Schueder
Petra Schwille
Ralf Jungmann
author_facet Florian Stehr
Johannes Stein
Florian Schueder
Petra Schwille
Ralf Jungmann
author_sort Florian Stehr
title Flat-top TIRF illumination boosts DNA-PAINT imaging and quantification
title_short Flat-top TIRF illumination boosts DNA-PAINT imaging and quantification
title_full Flat-top TIRF illumination boosts DNA-PAINT imaging and quantification
title_fullStr Flat-top TIRF illumination boosts DNA-PAINT imaging and quantification
title_full_unstemmed Flat-top TIRF illumination boosts DNA-PAINT imaging and quantification
title_sort flat-top tirf illumination boosts dna-paint imaging and quantification
publisher Nature Portfolio
publishDate 2019
url https://doaj.org/article/ead3c03afa5a468fbe0ee0e7061d0a7a
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AT johannesstein flattoptirfilluminationboostsdnapaintimagingandquantification
AT florianschueder flattoptirfilluminationboostsdnapaintimagingandquantification
AT petraschwille flattoptirfilluminationboostsdnapaintimagingandquantification
AT ralfjungmann flattoptirfilluminationboostsdnapaintimagingandquantification
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