Exploring the Diversity and Biotechnological Potential of Cultured and Uncultured Coral-Associated Bacteria

Coral-associated microbes are crucial for the biology of their hosts, contributing to nutrient cycling, adaptation, mitigation of toxic compounds, and biological control of pathogens. Natural products from coral-associated micro-organisms (CAM) may possess unique traits. Despite this, the use of CAM...

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Autores principales:	Caren Leite Spindola Vilela, Helena Dias Müller Villela, Caio Tavora Coelho da Costa Rachid, Flávia Lima do Carmo, Alane Beatriz Vermelho, Raquel Silva Peixoto
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	coral-associated micro-organisms (CAM) microbial diversity biotechnology enzymes coral reefs hydrolases Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/eadc8fdd381c4ee4adbb6b557dcb8ccd
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spelling	oai:doaj.org-article:eadc8fdd381c4ee4adbb6b557dcb8ccd2021-11-25T18:24:26ZExploring the Diversity and Biotechnological Potential of Cultured and Uncultured Coral-Associated Bacteria10.3390/microorganisms91122352076-2607https://doaj.org/article/eadc8fdd381c4ee4adbb6b557dcb8ccd2021-10-01T00:00:00Zhttps://www.mdpi.com/2076-2607/9/11/2235https://doaj.org/toc/2076-2607Coral-associated microbes are crucial for the biology of their hosts, contributing to nutrient cycling, adaptation, mitigation of toxic compounds, and biological control of pathogens. Natural products from coral-associated micro-organisms (CAM) may possess unique traits. Despite this, the use of CAM for biotechnological purposes has not yet been adequately explored. Here, we investigated the production of commercially important enzymes by 37 strains of bacteria isolated from the coral species <i>Mussismilia braziliensis</i>, <i>Millepora alcicornis</i>, and <i>Porites astreoides</i>. In-vitro enzymatic assays showed that up to 56% of the isolates produced at least one of the seven enzymes screened (lipase, caseinase, keratinase, cellulase, chitinase, amylase, and gelatinase); one strain, identified as <i>Bacillus amyloliquefaciens</i> produced all these enzymes. Additionally, coral species-specific cultured and uncultured microbial communities were identified. The phylum Firmicutes predominated among the isolates, including the genera <i>Exiguobacterium, Bacillus</i>, and <i>Halomonas</i>, among others. Next-generation sequencing and bacteria culturing produced similar but also complementary data, with certain genera detected only by one or the other method. Our results demonstrate the importance of exploring different coral species as sources of specific micro-organisms of biotechnological and industrial interest, at the same time reinforcing the economic and ecological importance of coral reefs as reservoirs of such diversity.Caren Leite Spindola VilelaHelena Dias Müller VillelaCaio Tavora Coelho da Costa RachidFlávia Lima do CarmoAlane Beatriz VermelhoRaquel Silva PeixotoMDPI AGarticlecoral-associated micro-organisms (CAM)microbial diversitybiotechnologyenzymescoral reefshydrolasesBiology (General)QH301-705.5ENMicroorganisms, Vol 9, Iss 2235, p 2235 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	coral-associated micro-organisms (CAM) microbial diversity biotechnology enzymes coral reefs hydrolases Biology (General) QH301-705.5
spellingShingle	coral-associated micro-organisms (CAM) microbial diversity biotechnology enzymes coral reefs hydrolases Biology (General) QH301-705.5 Caren Leite Spindola Vilela Helena Dias Müller Villela Caio Tavora Coelho da Costa Rachid Flávia Lima do Carmo Alane Beatriz Vermelho Raquel Silva Peixoto Exploring the Diversity and Biotechnological Potential of Cultured and Uncultured Coral-Associated Bacteria
description	Coral-associated microbes are crucial for the biology of their hosts, contributing to nutrient cycling, adaptation, mitigation of toxic compounds, and biological control of pathogens. Natural products from coral-associated micro-organisms (CAM) may possess unique traits. Despite this, the use of CAM for biotechnological purposes has not yet been adequately explored. Here, we investigated the production of commercially important enzymes by 37 strains of bacteria isolated from the coral species <i>Mussismilia braziliensis</i>, <i>Millepora alcicornis</i>, and <i>Porites astreoides</i>. In-vitro enzymatic assays showed that up to 56% of the isolates produced at least one of the seven enzymes screened (lipase, caseinase, keratinase, cellulase, chitinase, amylase, and gelatinase); one strain, identified as <i>Bacillus amyloliquefaciens</i> produced all these enzymes. Additionally, coral species-specific cultured and uncultured microbial communities were identified. The phylum Firmicutes predominated among the isolates, including the genera <i>Exiguobacterium, Bacillus</i>, and <i>Halomonas</i>, among others. Next-generation sequencing and bacteria culturing produced similar but also complementary data, with certain genera detected only by one or the other method. Our results demonstrate the importance of exploring different coral species as sources of specific micro-organisms of biotechnological and industrial interest, at the same time reinforcing the economic and ecological importance of coral reefs as reservoirs of such diversity.
format	article
author	Caren Leite Spindola Vilela Helena Dias Müller Villela Caio Tavora Coelho da Costa Rachid Flávia Lima do Carmo Alane Beatriz Vermelho Raquel Silva Peixoto
author_facet	Caren Leite Spindola Vilela Helena Dias Müller Villela Caio Tavora Coelho da Costa Rachid Flávia Lima do Carmo Alane Beatriz Vermelho Raquel Silva Peixoto
author_sort	Caren Leite Spindola Vilela
title	Exploring the Diversity and Biotechnological Potential of Cultured and Uncultured Coral-Associated Bacteria
title_short	Exploring the Diversity and Biotechnological Potential of Cultured and Uncultured Coral-Associated Bacteria
title_full	Exploring the Diversity and Biotechnological Potential of Cultured and Uncultured Coral-Associated Bacteria
title_fullStr	Exploring the Diversity and Biotechnological Potential of Cultured and Uncultured Coral-Associated Bacteria
title_full_unstemmed	Exploring the Diversity and Biotechnological Potential of Cultured and Uncultured Coral-Associated Bacteria
title_sort	exploring the diversity and biotechnological potential of cultured and uncultured coral-associated bacteria
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/eadc8fdd381c4ee4adbb6b557dcb8ccd
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Exploring the Diversity and Biotechnological Potential of Cultured and Uncultured Coral-Associated Bacteria

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