Generation of a DKK2 homozygous knockout human embryonic stem cell line using the CRISPR/Cas9 system

Dickkopf-2 (DKK2) is an antagonist of canonical Wnt signaling, which is involved in various biological processes of development, such as epidermal appendage formation and eye development. To identify underlying effects of DKK2 during embryonic development, we generated a DKK2 homozygous knockout hum...

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Autores principales:	Wang Zhang, Yuming Li, Li Wang, Tao Xin, Canwei Zhang
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/eb03646ec9154b79bedcec1a95cffb31
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spelling	oai:doaj.org-article:eb03646ec9154b79bedcec1a95cffb312021-12-02T05:00:19ZGeneration of a DKK2 homozygous knockout human embryonic stem cell line using the CRISPR/Cas9 system1873-506110.1016/j.scr.2021.102611https://doaj.org/article/eb03646ec9154b79bedcec1a95cffb312021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S187350612100458Xhttps://doaj.org/toc/1873-5061Dickkopf-2 (DKK2) is an antagonist of canonical Wnt signaling, which is involved in various biological processes of development, such as epidermal appendage formation and eye development. To identify underlying effects of DKK2 during embryonic development, we generated a DKK2 homozygous knockout human embryonic stem cell (hESC) line through the CRISPR/Cas9 genome-editing technology. This cell line, which maintains normal stem cell morphology and stably expresses pluripotent markers, could provide an ideal platform for exploring the role of DKK2 in embryonic development. In addition, Zeocin selection combined with tiny clone picking might be a highly efficient way to generate gene-knockout hESC lines.Wang ZhangYuming LiLi WangTao XinCanwei ZhangElsevierarticleBiology (General)QH301-705.5ENStem Cell Research, Vol 57, Iss , Pp 102611- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Biology (General) QH301-705.5
spellingShingle	Biology (General) QH301-705.5 Wang Zhang Yuming Li Li Wang Tao Xin Canwei Zhang Generation of a DKK2 homozygous knockout human embryonic stem cell line using the CRISPR/Cas9 system
description	Dickkopf-2 (DKK2) is an antagonist of canonical Wnt signaling, which is involved in various biological processes of development, such as epidermal appendage formation and eye development. To identify underlying effects of DKK2 during embryonic development, we generated a DKK2 homozygous knockout human embryonic stem cell (hESC) line through the CRISPR/Cas9 genome-editing technology. This cell line, which maintains normal stem cell morphology and stably expresses pluripotent markers, could provide an ideal platform for exploring the role of DKK2 in embryonic development. In addition, Zeocin selection combined with tiny clone picking might be a highly efficient way to generate gene-knockout hESC lines.
format	article
author	Wang Zhang Yuming Li Li Wang Tao Xin Canwei Zhang
author_facet	Wang Zhang Yuming Li Li Wang Tao Xin Canwei Zhang
author_sort	Wang Zhang
title	Generation of a DKK2 homozygous knockout human embryonic stem cell line using the CRISPR/Cas9 system
title_short	Generation of a DKK2 homozygous knockout human embryonic stem cell line using the CRISPR/Cas9 system
title_full	Generation of a DKK2 homozygous knockout human embryonic stem cell line using the CRISPR/Cas9 system
title_fullStr	Generation of a DKK2 homozygous knockout human embryonic stem cell line using the CRISPR/Cas9 system
title_full_unstemmed	Generation of a DKK2 homozygous knockout human embryonic stem cell line using the CRISPR/Cas9 system
title_sort	generation of a dkk2 homozygous knockout human embryonic stem cell line using the crispr/cas9 system
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/eb03646ec9154b79bedcec1a95cffb31
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Generation of a DKK2 homozygous knockout human embryonic stem cell line using the CRISPR/Cas9 system

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