Multi-Factor Regulation of the Master Modulator LeuO for the Cyclic-(Phe-Pro) Signaling Pathway in Vibrio vulnificus

Abstract LeuO plays the role of a master regulator in the cyclic-L-phenylalanine-L-proline (cFP)-dependent signaling pathway in Vibrio vulnificus. cFP, as shown through isothermal titration calorimetry analysis, binds specifically to the periplasmic domain of ToxR. Binding of cFP triggers a change i...

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Autores principales:	Na-Young Park, In Hwang Kim, Yancheng Wen, Keun-Woo Lee, Sora Lee, Jeong-A Kim, Kwang-Hwan Jung, Kyu-Ho Lee, Kun-Soo Kim
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2019
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/ebc8fdc1b34548bcb931318de56002b1
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spelling	oai:doaj.org-article:ebc8fdc1b34548bcb931318de56002b12021-12-02T13:34:54ZMulti-Factor Regulation of the Master Modulator LeuO for the Cyclic-(Phe-Pro) Signaling Pathway in Vibrio vulnificus10.1038/s41598-019-56855-42045-2322https://doaj.org/article/ebc8fdc1b34548bcb931318de56002b12019-12-01T00:00:00Zhttps://doi.org/10.1038/s41598-019-56855-4https://doaj.org/toc/2045-2322Abstract LeuO plays the role of a master regulator in the cyclic-L-phenylalanine-L-proline (cFP)-dependent signaling pathway in Vibrio vulnificus. cFP, as shown through isothermal titration calorimetry analysis, binds specifically to the periplasmic domain of ToxR. Binding of cFP triggers a change in the cytoplasmic domain of ToxR, which then activates transcription of leuO encoding a LysR-type regulator. LeuO binds to the region upstream of its own coding sequence, inhibiting its own transcription and maintaining a controlled level of expression. A five-bp deletion in this region abolished expression of LeuO, but a ten-bp deletion did not, suggesting that a DNA bending mechanism is involved in the regulation. Furthermore, binding of RNA polymerase was significantly lower both in the deletion of the ToxR binding site and in the five-bp deletion, but not in the ten-bp deletion, as shown in pull-down assays using an antibody against RNA polymerase subunit α. In summary, multiple factors are involved in control of the expression of LeuO, a master regulator that orchestrates downstream regulators to modulate factors required for survival and pathogenicity of the pathogen.Na-Young ParkIn Hwang KimYancheng WenKeun-Woo LeeSora LeeJeong-A KimKwang-Hwan JungKyu-Ho LeeKun-Soo KimNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 9, Iss 1, Pp 1-14 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Na-Young Park In Hwang Kim Yancheng Wen Keun-Woo Lee Sora Lee Jeong-A Kim Kwang-Hwan Jung Kyu-Ho Lee Kun-Soo Kim Multi-Factor Regulation of the Master Modulator LeuO for the Cyclic-(Phe-Pro) Signaling Pathway in Vibrio vulnificus
description	Abstract LeuO plays the role of a master regulator in the cyclic-L-phenylalanine-L-proline (cFP)-dependent signaling pathway in Vibrio vulnificus. cFP, as shown through isothermal titration calorimetry analysis, binds specifically to the periplasmic domain of ToxR. Binding of cFP triggers a change in the cytoplasmic domain of ToxR, which then activates transcription of leuO encoding a LysR-type regulator. LeuO binds to the region upstream of its own coding sequence, inhibiting its own transcription and maintaining a controlled level of expression. A five-bp deletion in this region abolished expression of LeuO, but a ten-bp deletion did not, suggesting that a DNA bending mechanism is involved in the regulation. Furthermore, binding of RNA polymerase was significantly lower both in the deletion of the ToxR binding site and in the five-bp deletion, but not in the ten-bp deletion, as shown in pull-down assays using an antibody against RNA polymerase subunit α. In summary, multiple factors are involved in control of the expression of LeuO, a master regulator that orchestrates downstream regulators to modulate factors required for survival and pathogenicity of the pathogen.
format	article
author	Na-Young Park In Hwang Kim Yancheng Wen Keun-Woo Lee Sora Lee Jeong-A Kim Kwang-Hwan Jung Kyu-Ho Lee Kun-Soo Kim
author_facet	Na-Young Park In Hwang Kim Yancheng Wen Keun-Woo Lee Sora Lee Jeong-A Kim Kwang-Hwan Jung Kyu-Ho Lee Kun-Soo Kim
author_sort	Na-Young Park
title	Multi-Factor Regulation of the Master Modulator LeuO for the Cyclic-(Phe-Pro) Signaling Pathway in Vibrio vulnificus
title_short	Multi-Factor Regulation of the Master Modulator LeuO for the Cyclic-(Phe-Pro) Signaling Pathway in Vibrio vulnificus
title_full	Multi-Factor Regulation of the Master Modulator LeuO for the Cyclic-(Phe-Pro) Signaling Pathway in Vibrio vulnificus
title_fullStr	Multi-Factor Regulation of the Master Modulator LeuO for the Cyclic-(Phe-Pro) Signaling Pathway in Vibrio vulnificus
title_full_unstemmed	Multi-Factor Regulation of the Master Modulator LeuO for the Cyclic-(Phe-Pro) Signaling Pathway in Vibrio vulnificus
title_sort	multi-factor regulation of the master modulator leuo for the cyclic-(phe-pro) signaling pathway in vibrio vulnificus
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/ebc8fdc1b34548bcb931318de56002b1
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Multi-Factor Regulation of the Master Modulator LeuO for the Cyclic-(Phe-Pro) Signaling Pathway in Vibrio vulnificus

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