Unraveling the functional role of DNA demethylation at specific promoters by targeted steric blockage of DNA methyltransferase with CRISPR/dCas9

The causal relationship between DNA demethylation and gene expression regulation has not yet been fully resolved. Here the authors develop a nuclease-dead Cas9 (dCas9) and gRNA site-specific targeting approach to physically block DNA methylation at specific promoters to cause DNA demethylation in ce...

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Main Authors:	Daniel M. Sapozhnikov, Moshe Szyf
Format:	article
Language:	EN
Published:	Nature Portfolio 2021
Subjects:	Science Q
Online Access:	https://doaj.org/article/ec5522b1496a4c3d90ef8ee39e17ce5a
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spelling	oai:doaj.org-article:ec5522b1496a4c3d90ef8ee39e17ce5a2021-12-02T19:16:51ZUnraveling the functional role of DNA demethylation at specific promoters by targeted steric blockage of DNA methyltransferase with CRISPR/dCas910.1038/s41467-021-25991-92041-1723https://doaj.org/article/ec5522b1496a4c3d90ef8ee39e17ce5a2021-09-01T00:00:00Zhttps://doi.org/10.1038/s41467-021-25991-9https://doaj.org/toc/2041-1723The causal relationship between DNA demethylation and gene expression regulation has not yet been fully resolved. Here the authors develop a nuclease-dead Cas9 (dCas9) and gRNA site-specific targeting approach to physically block DNA methylation at specific promoters to cause DNA demethylation in cells and tackle this question.Daniel M. SapozhnikovMoshe SzyfNature PortfolioarticleScienceQENNature Communications, Vol 12, Iss 1, Pp 1-26 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Daniel M. Sapozhnikov Moshe Szyf Unraveling the functional role of DNA demethylation at specific promoters by targeted steric blockage of DNA methyltransferase with CRISPR/dCas9
description	The causal relationship between DNA demethylation and gene expression regulation has not yet been fully resolved. Here the authors develop a nuclease-dead Cas9 (dCas9) and gRNA site-specific targeting approach to physically block DNA methylation at specific promoters to cause DNA demethylation in cells and tackle this question.
format	article
author	Daniel M. Sapozhnikov Moshe Szyf
author_facet	Daniel M. Sapozhnikov Moshe Szyf
author_sort	Daniel M. Sapozhnikov
title	Unraveling the functional role of DNA demethylation at specific promoters by targeted steric blockage of DNA methyltransferase with CRISPR/dCas9
title_short	Unraveling the functional role of DNA demethylation at specific promoters by targeted steric blockage of DNA methyltransferase with CRISPR/dCas9
title_full	Unraveling the functional role of DNA demethylation at specific promoters by targeted steric blockage of DNA methyltransferase with CRISPR/dCas9
title_fullStr	Unraveling the functional role of DNA demethylation at specific promoters by targeted steric blockage of DNA methyltransferase with CRISPR/dCas9
title_full_unstemmed	Unraveling the functional role of DNA demethylation at specific promoters by targeted steric blockage of DNA methyltransferase with CRISPR/dCas9
title_sort	unraveling the functional role of dna demethylation at specific promoters by targeted steric blockage of dna methyltransferase with crispr/dcas9
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/ec5522b1496a4c3d90ef8ee39e17ce5a
work_keys_str_mv	AT danielmsapozhnikov unravelingthefunctionalroleofdnademethylationatspecificpromotersbytargetedstericblockageofdnamethyltransferasewithcrisprdcas9 AT mosheszyf unravelingthefunctionalroleofdnademethylationatspecificpromotersbytargetedstericblockageofdnamethyltransferasewithcrisprdcas9
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Unraveling the functional role of DNA demethylation at specific promoters by targeted steric blockage of DNA methyltransferase with CRISPR/dCas9

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