A Highly Sensitive GFP Activation Assay for Detection of DNA Cleavage in Cells

CRISPR/Cas9 nucleases hold great potential for gene therapy, but they frequently induce unwanted off-target cleavage. We previously developed a GFP activation assay for detection of DNA cleavage in cells. Here, we demonstrate two novel applications of this assay. First, we use this assay to confirm...

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Autores principales: Ziying Hu, Chengdong Zhang, Daqi Wang, Siqi Gao, Sang-Ging Ong, Yongming Wang, Wei V. Zheng
Formato: article
Lenguaje:EN
Publicado: Frontiers Media S.A. 2021
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Acceso en línea:https://doaj.org/article/ed723177ffba47588f6be8e71deee98e
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