Genetic and Phenotypic Diversity of Morganella morganii Isolated From Cheese

The bacterium Morganella morganii can produce the biogenic amines (BA) cadaverine, putrescine, and histamine in vitro and is responsible for high histamine concentrations in fish products. These BA can have toxic effects upon ingestion and are undesired in food. The purpose of this study was to char...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Lorenz Timo Ryser, Emmanuelle Arias-Roth, Vincent Perreten, Stefan Irmler, Rémy Bruggmann
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	Morganella morganii biogenic amines lysine decarboxylase antibiotic resistances histamine cadaverine Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/edbf7cd3480440c197aed36fbaeb9297
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:edbf7cd3480440c197aed36fbaeb9297
record_format	dspace
spelling	oai:doaj.org-article:edbf7cd3480440c197aed36fbaeb92972021-11-17T13:48:01ZGenetic and Phenotypic Diversity of Morganella morganii Isolated From Cheese1664-302X10.3389/fmicb.2021.738492https://doaj.org/article/edbf7cd3480440c197aed36fbaeb92972021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fmicb.2021.738492/fullhttps://doaj.org/toc/1664-302XThe bacterium Morganella morganii can produce the biogenic amines (BA) cadaverine, putrescine, and histamine in vitro and is responsible for high histamine concentrations in fish products. These BA can have toxic effects upon ingestion and are undesired in food. The purpose of this study was to characterize the phenotype and genotype of 11 M. morganii isolated from cheese in regard to the BA formation. In addition, we investigated the phylogeny, trehalose fermentation ability, and antibiotic resistance of the cheese isolates. To do so, we sequenced their genomes using both long and short read technologies. Due to the presence of the trehalose operon and the ability to ferment trehalose, the cheese isolates can be assigned to the subsp. sibonii. Comparative genomics with public available M. morganii genomes shows that the genomes of the cheese isolates cluster together with other subsp. sibonii genomes. All genomes between subsp. morganii and subsp. sibonii are separated by an average nucleotide identity (ANI) of less than 95.0%. Therefore, the subspecies could represent two distinct species. Nine of the strains decarboxylated lysine yielding cadaverine in vitro. This metabolic activity is linked to a previously unknown gene cluster comprising genes encoding a lysine-tRNA ligase (lysS), an HTH-transcriptional regulator (argP), a cadaverine-lysine antiporter (cadB), and a lysine decarboxylase (cadA). The formation of putrescine is linked to the speF gene encoding an ornithine decarboxylase. The gene is disrupted in five strains by an insertion sequence, and these strains only exhibit a weak putrescine production. Antimicrobial susceptibility profiling revealed that all cheese strains are resistant to tetracycline, chloramphenicol, tigecycline, colistin, and ampicillin. These phenotypes, except for colistin which is intrinsic, could be linked to antimicrobial resistance genes located on the chromosome.Lorenz Timo RyserLorenz Timo RyserEmmanuelle Arias-RothVincent PerretenStefan IrmlerRémy BruggmannFrontiers Media S.A.articleMorganella morganiibiogenic amineslysine decarboxylaseantibiotic resistanceshistaminecadaverineMicrobiologyQR1-502ENFrontiers in Microbiology, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Morganella morganii biogenic amines lysine decarboxylase antibiotic resistances histamine cadaverine Microbiology QR1-502
spellingShingle	Morganella morganii biogenic amines lysine decarboxylase antibiotic resistances histamine cadaverine Microbiology QR1-502 Lorenz Timo Ryser Lorenz Timo Ryser Emmanuelle Arias-Roth Vincent Perreten Stefan Irmler Rémy Bruggmann Genetic and Phenotypic Diversity of Morganella morganii Isolated From Cheese
description	The bacterium Morganella morganii can produce the biogenic amines (BA) cadaverine, putrescine, and histamine in vitro and is responsible for high histamine concentrations in fish products. These BA can have toxic effects upon ingestion and are undesired in food. The purpose of this study was to characterize the phenotype and genotype of 11 M. morganii isolated from cheese in regard to the BA formation. In addition, we investigated the phylogeny, trehalose fermentation ability, and antibiotic resistance of the cheese isolates. To do so, we sequenced their genomes using both long and short read technologies. Due to the presence of the trehalose operon and the ability to ferment trehalose, the cheese isolates can be assigned to the subsp. sibonii. Comparative genomics with public available M. morganii genomes shows that the genomes of the cheese isolates cluster together with other subsp. sibonii genomes. All genomes between subsp. morganii and subsp. sibonii are separated by an average nucleotide identity (ANI) of less than 95.0%. Therefore, the subspecies could represent two distinct species. Nine of the strains decarboxylated lysine yielding cadaverine in vitro. This metabolic activity is linked to a previously unknown gene cluster comprising genes encoding a lysine-tRNA ligase (lysS), an HTH-transcriptional regulator (argP), a cadaverine-lysine antiporter (cadB), and a lysine decarboxylase (cadA). The formation of putrescine is linked to the speF gene encoding an ornithine decarboxylase. The gene is disrupted in five strains by an insertion sequence, and these strains only exhibit a weak putrescine production. Antimicrobial susceptibility profiling revealed that all cheese strains are resistant to tetracycline, chloramphenicol, tigecycline, colistin, and ampicillin. These phenotypes, except for colistin which is intrinsic, could be linked to antimicrobial resistance genes located on the chromosome.
format	article
author	Lorenz Timo Ryser Lorenz Timo Ryser Emmanuelle Arias-Roth Vincent Perreten Stefan Irmler Rémy Bruggmann
author_facet	Lorenz Timo Ryser Lorenz Timo Ryser Emmanuelle Arias-Roth Vincent Perreten Stefan Irmler Rémy Bruggmann
author_sort	Lorenz Timo Ryser
title	Genetic and Phenotypic Diversity of Morganella morganii Isolated From Cheese
title_short	Genetic and Phenotypic Diversity of Morganella morganii Isolated From Cheese
title_full	Genetic and Phenotypic Diversity of Morganella morganii Isolated From Cheese
title_fullStr	Genetic and Phenotypic Diversity of Morganella morganii Isolated From Cheese
title_full_unstemmed	Genetic and Phenotypic Diversity of Morganella morganii Isolated From Cheese
title_sort	genetic and phenotypic diversity of morganella morganii isolated from cheese
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/edbf7cd3480440c197aed36fbaeb9297
work_keys_str_mv	AT lorenztimoryser geneticandphenotypicdiversityofmorganellamorganiiisolatedfromcheese AT lorenztimoryser geneticandphenotypicdiversityofmorganellamorganiiisolatedfromcheese AT emmanuelleariasroth geneticandphenotypicdiversityofmorganellamorganiiisolatedfromcheese AT vincentperreten geneticandphenotypicdiversityofmorganellamorganiiisolatedfromcheese AT stefanirmler geneticandphenotypicdiversityofmorganellamorganiiisolatedfromcheese AT remybruggmann geneticandphenotypicdiversityofmorganellamorganiiisolatedfromcheese
_version_	1718425582397554688

Genetic and Phenotypic Diversity of Morganella morganii Isolated From Cheese

Ejemplares similares