Grass pea natural variation reveals oligogenic resistance to Fusarium oxysporum f. sp. pisi

Abstract Grass pea (Lathyrus sativus L.) is an annual legume species, phylogenetically close to pea (Pisum sativum L.), that may be infected by Fusarium oxysporum f. sp. pisi (Fop), the causal agent of fusarium wilt in peas with vast worldwide yield losses. A range of responses varying from high res...

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Autores principales:	Ana Margarida Sampaio, Mara Lisa Alves, Priscila Pereira, Ehsan Valiollahi, Carmen Santos, Zlatko Šatović, Diego Rubiales, Susana de Sousa Araújo, Fred van Eeuwijk, Maria Carlota Vaz Patto
Formato:	article
Lenguaje:	EN
Publicado:	Wiley 2021
Materias:	Plant culture SB1-1110 Genetics QH426-470
Acceso en línea:	https://doaj.org/article/ee017fb64e234e95b6335df1cb5eb005
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spelling	oai:doaj.org-article:ee017fb64e234e95b6335df1cb5eb0052021-12-05T07:50:12ZGrass pea natural variation reveals oligogenic resistance to Fusarium oxysporum f. sp. pisi1940-337210.1002/tpg2.20154https://doaj.org/article/ee017fb64e234e95b6335df1cb5eb0052021-11-01T00:00:00Zhttps://doi.org/10.1002/tpg2.20154https://doaj.org/toc/1940-3372Abstract Grass pea (Lathyrus sativus L.) is an annual legume species, phylogenetically close to pea (Pisum sativum L.), that may be infected by Fusarium oxysporum f. sp. pisi (Fop), the causal agent of fusarium wilt in peas with vast worldwide yield losses. A range of responses varying from high resistance to susceptibility to this pathogen has been reported in grass pea germplasm. Nevertheless, the genetic basis of that diversity of responses is still unknown, hampering its breeding exploitation. To identify genomic regions controlling grass pea resistance to fusarium wilt, a genome‐wide association study approach was applied on a grass pea worldwide collection of accessions inoculated with Fop race 2. Disease responses were scored in this collection that was also subjected to high‐throughput based single nucleotide polymorphisms (SNP) screening through genotyping‐by‐sequencing. A total of 5,651 high‐quality SNPs were considered for association mapping analysis, performed using mixed linear models accounting for population structure. Because of the absence of a fully assembled grass pea reference genome, SNP markers’ genomic positions were retrieved from the pea's reference genome v1a. In total, 17 genomic regions were associated with three fusarium wilt response traits in grass pea, anticipating an oligogenic control. Seven of these regions were located on pea chromosomes 1, 6, and 7. The candidate genes underlying these regions were putatively involved in secondary and amino acid metabolism, RNA (regulation of transcription), transport, and development. This study revealed important fusarium wilt resistance favorable grass pea SNP alleles, allowing the development of molecular tools for precision disease resistance breeding.Ana Margarida SampaioMara Lisa AlvesPriscila PereiraEhsan ValiollahiCarmen SantosZlatko ŠatovićDiego RubialesSusana de Sousa AraújoFred van EeuwijkMaria Carlota Vaz PattoWileyarticlePlant cultureSB1-1110GeneticsQH426-470ENThe Plant Genome, Vol 14, Iss 3, Pp n/a-n/a (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Plant culture SB1-1110 Genetics QH426-470
spellingShingle	Plant culture SB1-1110 Genetics QH426-470 Ana Margarida Sampaio Mara Lisa Alves Priscila Pereira Ehsan Valiollahi Carmen Santos Zlatko Šatović Diego Rubiales Susana de Sousa Araújo Fred van Eeuwijk Maria Carlota Vaz Patto Grass pea natural variation reveals oligogenic resistance to Fusarium oxysporum f. sp. pisi
description	Abstract Grass pea (Lathyrus sativus L.) is an annual legume species, phylogenetically close to pea (Pisum sativum L.), that may be infected by Fusarium oxysporum f. sp. pisi (Fop), the causal agent of fusarium wilt in peas with vast worldwide yield losses. A range of responses varying from high resistance to susceptibility to this pathogen has been reported in grass pea germplasm. Nevertheless, the genetic basis of that diversity of responses is still unknown, hampering its breeding exploitation. To identify genomic regions controlling grass pea resistance to fusarium wilt, a genome‐wide association study approach was applied on a grass pea worldwide collection of accessions inoculated with Fop race 2. Disease responses were scored in this collection that was also subjected to high‐throughput based single nucleotide polymorphisms (SNP) screening through genotyping‐by‐sequencing. A total of 5,651 high‐quality SNPs were considered for association mapping analysis, performed using mixed linear models accounting for population structure. Because of the absence of a fully assembled grass pea reference genome, SNP markers’ genomic positions were retrieved from the pea's reference genome v1a. In total, 17 genomic regions were associated with three fusarium wilt response traits in grass pea, anticipating an oligogenic control. Seven of these regions were located on pea chromosomes 1, 6, and 7. The candidate genes underlying these regions were putatively involved in secondary and amino acid metabolism, RNA (regulation of transcription), transport, and development. This study revealed important fusarium wilt resistance favorable grass pea SNP alleles, allowing the development of molecular tools for precision disease resistance breeding.
format	article
author	Ana Margarida Sampaio Mara Lisa Alves Priscila Pereira Ehsan Valiollahi Carmen Santos Zlatko Šatović Diego Rubiales Susana de Sousa Araújo Fred van Eeuwijk Maria Carlota Vaz Patto
author_facet	Ana Margarida Sampaio Mara Lisa Alves Priscila Pereira Ehsan Valiollahi Carmen Santos Zlatko Šatović Diego Rubiales Susana de Sousa Araújo Fred van Eeuwijk Maria Carlota Vaz Patto
author_sort	Ana Margarida Sampaio
title	Grass pea natural variation reveals oligogenic resistance to Fusarium oxysporum f. sp. pisi
title_short	Grass pea natural variation reveals oligogenic resistance to Fusarium oxysporum f. sp. pisi
title_full	Grass pea natural variation reveals oligogenic resistance to Fusarium oxysporum f. sp. pisi
title_fullStr	Grass pea natural variation reveals oligogenic resistance to Fusarium oxysporum f. sp. pisi
title_full_unstemmed	Grass pea natural variation reveals oligogenic resistance to Fusarium oxysporum f. sp. pisi
title_sort	grass pea natural variation reveals oligogenic resistance to fusarium oxysporum f. sp. pisi
publisher	Wiley
publishDate	2021
url	https://doaj.org/article/ee017fb64e234e95b6335df1cb5eb005
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Grass pea natural variation reveals oligogenic resistance to Fusarium oxysporum f. sp. pisi

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