Long non-coding RNA (lncRNA) nuclear enriched abundant transcript 1 (NEAT1) promotes the inflammation and apoptosis of otitis media with effusion through targeting microRNA (miR)-495 and activation of p38 MAPK signaling pathway

Long non-coding RNA (lncRNA) nuclear enriched abundant transcript 1 (NEAT1) promotes the inflammation and apoptosis of otitis media with effusion through targeting microRNA (miR)-495 and activation of p38 MAPK signaling pathway

Long non-coding RNA (lncRNA) plays a vital role in human inflammatory diseases. Our study aimed to investigate the function of lncRNA nuclear-enriched abundant transcript 1 (NEAT1) in otitis media with effusion (OME). The mRNA levels of NEAT1 and miR-495 were measured by RT-qPCR. The protein levels...

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Autores principales: Yajuan Hu, Hongjun Dong, Jingjing Huang, Jie Huang, Dandan Tao, Chao Huang, Lei Hu, Hao Xu, Yan Sun
Formato: article
Lenguaje:EN
Publicado: Taylor & Francis Group 2021
Materias:
otitis media with effusion
neat1
mir-495
mapk
inflammation
Biotechnology
TP248.13-248.65
Acceso en línea:https://doaj.org/article/ee9e209be85a4073b4f64e9c0ba68562
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spelling oai:doaj.org-article:ee9e209be85a4073b4f64e9c0ba685622021-11-04T15:51:53ZLong non-coding RNA (lncRNA) nuclear enriched abundant transcript 1 (NEAT1) promotes the inflammation and apoptosis of otitis media with effusion through targeting microRNA (miR)-495 and activation of p38 MAPK signaling pathway2165-59792165-598710.1080/21655979.2021.1982842https://doaj.org/article/ee9e209be85a4073b4f64e9c0ba685622021-01-01T00:00:00Zhttp://dx.doi.org/10.1080/21655979.2021.1982842https://doaj.org/toc/2165-5979https://doaj.org/toc/2165-5987Long non-coding RNA (lncRNA) plays a vital role in human inflammatory diseases. Our study aimed to investigate the function of lncRNA nuclear-enriched abundant transcript 1 (NEAT1) in otitis media with effusion (OME). The mRNA levels of NEAT1 and miR-495 were measured by RT-qPCR. The protein levels of p38 MAPK were detected by western blot. The levels of inflammatory cytokines were examined by ELISA. CCK-8 and flow cytometry assays were used to evaluate the cell viability and apoptosis, respectively. The interaction between NEAT1 and miR-495 was determined by luciferase reporter and RIP assays. NEAT1 was highly expressed in OME, and silencing of NEAT1 facilitated the cell proliferation and suppressed levels of inflammatory cytokines and cell apoptosis in LPS-induced HMEECs. Moreover, miR-495 was confirmed as a downstream target of NEAT1. Functional assays revealed that NEAT1 promoted the OME by targeting miR-495. It was further demonstrated that NEAT1 could activate the p38 MAPK signaling pathway by regulating miR-495, and the p38 MAPK inhibitor restored the effects of NEAT1 overexpression on the inflammation levels, cell proliferation, and apoptosis. Our study revealed that lncRNA NEAT1 served as a ceRNA to activate p38 MAPK signaling by targeting miR-495 in OME, which may offer a new target for OME treatment.Yajuan HuHongjun DongJingjing HuangJie HuangDandan TaoChao HuangLei HuHao XuYan SunTaylor & Francis Grouparticleotitis media with effusionneat1mir-495mapkinflammationBiotechnologyTP248.13-248.65ENBioengineered, Vol 12, Iss 1, Pp 8080-8088 (2021)
institution DOAJ
collection DOAJ
language EN
topic otitis media with effusion
neat1
mir-495
mapk
inflammation
Biotechnology
TP248.13-248.65
spellingShingle otitis media with effusion
neat1
mir-495
mapk
inflammation
Biotechnology
TP248.13-248.65
Yajuan Hu
Hongjun Dong
Jingjing Huang
Jie Huang
Dandan Tao
Chao Huang
Lei Hu
Hao Xu
Yan Sun
Long non-coding RNA (lncRNA) nuclear enriched abundant transcript 1 (NEAT1) promotes the inflammation and apoptosis of otitis media with effusion through targeting microRNA (miR)-495 and activation of p38 MAPK signaling pathway
description Long non-coding RNA (lncRNA) plays a vital role in human inflammatory diseases. Our study aimed to investigate the function of lncRNA nuclear-enriched abundant transcript 1 (NEAT1) in otitis media with effusion (OME). The mRNA levels of NEAT1 and miR-495 were measured by RT-qPCR. The protein levels of p38 MAPK were detected by western blot. The levels of inflammatory cytokines were examined by ELISA. CCK-8 and flow cytometry assays were used to evaluate the cell viability and apoptosis, respectively. The interaction between NEAT1 and miR-495 was determined by luciferase reporter and RIP assays. NEAT1 was highly expressed in OME, and silencing of NEAT1 facilitated the cell proliferation and suppressed levels of inflammatory cytokines and cell apoptosis in LPS-induced HMEECs. Moreover, miR-495 was confirmed as a downstream target of NEAT1. Functional assays revealed that NEAT1 promoted the OME by targeting miR-495. It was further demonstrated that NEAT1 could activate the p38 MAPK signaling pathway by regulating miR-495, and the p38 MAPK inhibitor restored the effects of NEAT1 overexpression on the inflammation levels, cell proliferation, and apoptosis. Our study revealed that lncRNA NEAT1 served as a ceRNA to activate p38 MAPK signaling by targeting miR-495 in OME, which may offer a new target for OME treatment.
format article
author Yajuan Hu
Hongjun Dong
Jingjing Huang
Jie Huang
Dandan Tao
Chao Huang
Lei Hu
Hao Xu
Yan Sun
author_facet Yajuan Hu
Hongjun Dong
Jingjing Huang
Jie Huang
Dandan Tao
Chao Huang
Lei Hu
Hao Xu
Yan Sun
author_sort Yajuan Hu
title Long non-coding RNA (lncRNA) nuclear enriched abundant transcript 1 (NEAT1) promotes the inflammation and apoptosis of otitis media with effusion through targeting microRNA (miR)-495 and activation of p38 MAPK signaling pathway
title_short Long non-coding RNA (lncRNA) nuclear enriched abundant transcript 1 (NEAT1) promotes the inflammation and apoptosis of otitis media with effusion through targeting microRNA (miR)-495 and activation of p38 MAPK signaling pathway
title_full Long non-coding RNA (lncRNA) nuclear enriched abundant transcript 1 (NEAT1) promotes the inflammation and apoptosis of otitis media with effusion through targeting microRNA (miR)-495 and activation of p38 MAPK signaling pathway
title_fullStr Long non-coding RNA (lncRNA) nuclear enriched abundant transcript 1 (NEAT1) promotes the inflammation and apoptosis of otitis media with effusion through targeting microRNA (miR)-495 and activation of p38 MAPK signaling pathway
title_full_unstemmed Long non-coding RNA (lncRNA) nuclear enriched abundant transcript 1 (NEAT1) promotes the inflammation and apoptosis of otitis media with effusion through targeting microRNA (miR)-495 and activation of p38 MAPK signaling pathway
title_sort long non-coding rna (lncrna) nuclear enriched abundant transcript 1 (neat1) promotes the inflammation and apoptosis of otitis media with effusion through targeting microrna (mir)-495 and activation of p38 mapk signaling pathway
publisher Taylor & Francis Group
publishDate 2021
url https://doaj.org/article/ee9e209be85a4073b4f64e9c0ba68562
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