Dual loss of human POLQ and LIG4 abolishes random integration

Homologous recombination mediated gene targeting is highly inefficient in human cells due to random integration events, Here the authors show that dual repression of polymerase θ and DNA ligase IV eliminate random integration events.

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Autores principales:	Shinta Saito, Ryo Maeda, Noritaka Adachi
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/ef54135e9bea42c6a525eac4bbb609dc
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spelling	oai:doaj.org-article:ef54135e9bea42c6a525eac4bbb609dc2021-12-02T15:38:38ZDual loss of human POLQ and LIG4 abolishes random integration10.1038/ncomms161122041-1723https://doaj.org/article/ef54135e9bea42c6a525eac4bbb609dc2017-07-01T00:00:00Zhttps://doi.org/10.1038/ncomms16112https://doaj.org/toc/2041-1723Homologous recombination mediated gene targeting is highly inefficient in human cells due to random integration events, Here the authors show that dual repression of polymerase θ and DNA ligase IV eliminate random integration events.Shinta SaitoRyo MaedaNoritaka AdachiNature PortfolioarticleScienceQENNature Communications, Vol 8, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Shinta Saito Ryo Maeda Noritaka Adachi Dual loss of human POLQ and LIG4 abolishes random integration
description	Homologous recombination mediated gene targeting is highly inefficient in human cells due to random integration events, Here the authors show that dual repression of polymerase θ and DNA ligase IV eliminate random integration events.
format	article
author	Shinta Saito Ryo Maeda Noritaka Adachi
author_facet	Shinta Saito Ryo Maeda Noritaka Adachi
author_sort	Shinta Saito
title	Dual loss of human POLQ and LIG4 abolishes random integration
title_short	Dual loss of human POLQ and LIG4 abolishes random integration
title_full	Dual loss of human POLQ and LIG4 abolishes random integration
title_fullStr	Dual loss of human POLQ and LIG4 abolishes random integration
title_full_unstemmed	Dual loss of human POLQ and LIG4 abolishes random integration
title_sort	dual loss of human polq and lig4 abolishes random integration
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/ef54135e9bea42c6a525eac4bbb609dc
work_keys_str_mv	AT shintasaito duallossofhumanpolqandlig4abolishesrandomintegration AT ryomaeda duallossofhumanpolqandlig4abolishesrandomintegration AT noritakaadachi duallossofhumanpolqandlig4abolishesrandomintegration
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Dual loss of human POLQ and LIG4 abolishes random integration

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