A masked initiation region in retinoblastoma protein regulates its proteasomal degradation

Human papilloma virus (HPV) E7 protein destabilizes the retinoblastoma protein (Rb) by inducing its ubiquitination in cervical cancer cells, however proteasomal degradation requires cleavage of Rb after Lys 810 and so far it has been unclear how Rb cleavage contributes to its degradation. Here, the...

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Autores principales:	Takuya Tomita, Jon M. Huibregtse, Andreas Matouschek
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/efa1fd80046b433d9c08b09e1c821f0f
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spelling	oai:doaj.org-article:efa1fd80046b433d9c08b09e1c821f0f2021-12-02T17:32:12ZA masked initiation region in retinoblastoma protein regulates its proteasomal degradation10.1038/s41467-020-16003-32041-1723https://doaj.org/article/efa1fd80046b433d9c08b09e1c821f0f2020-04-01T00:00:00Zhttps://doi.org/10.1038/s41467-020-16003-3https://doaj.org/toc/2041-1723Human papilloma virus (HPV) E7 protein destabilizes the retinoblastoma protein (Rb) by inducing its ubiquitination in cervical cancer cells, however proteasomal degradation requires cleavage of Rb after Lys 810 and so far it has been unclear how Rb cleavage contributes to its degradation. Here, the authors combine cell based and in vitro assays and show that calpain cleavage exposes a region in Rb that is recognized by the proteasome, leading to rapid proteolysis of Rb, whereas the proteasome cannot initiate degradation efficiently on full-length Rb.Takuya TomitaJon M. HuibregtseAndreas MatouschekNature PortfolioarticleScienceQENNature Communications, Vol 11, Iss 1, Pp 1-8 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Takuya Tomita Jon M. Huibregtse Andreas Matouschek A masked initiation region in retinoblastoma protein regulates its proteasomal degradation
description	Human papilloma virus (HPV) E7 protein destabilizes the retinoblastoma protein (Rb) by inducing its ubiquitination in cervical cancer cells, however proteasomal degradation requires cleavage of Rb after Lys 810 and so far it has been unclear how Rb cleavage contributes to its degradation. Here, the authors combine cell based and in vitro assays and show that calpain cleavage exposes a region in Rb that is recognized by the proteasome, leading to rapid proteolysis of Rb, whereas the proteasome cannot initiate degradation efficiently on full-length Rb.
format	article
author	Takuya Tomita Jon M. Huibregtse Andreas Matouschek
author_facet	Takuya Tomita Jon M. Huibregtse Andreas Matouschek
author_sort	Takuya Tomita
title	A masked initiation region in retinoblastoma protein regulates its proteasomal degradation
title_short	A masked initiation region in retinoblastoma protein regulates its proteasomal degradation
title_full	A masked initiation region in retinoblastoma protein regulates its proteasomal degradation
title_fullStr	A masked initiation region in retinoblastoma protein regulates its proteasomal degradation
title_full_unstemmed	A masked initiation region in retinoblastoma protein regulates its proteasomal degradation
title_sort	masked initiation region in retinoblastoma protein regulates its proteasomal degradation
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/efa1fd80046b433d9c08b09e1c821f0f
work_keys_str_mv	AT takuyatomita amaskedinitiationregioninretinoblastomaproteinregulatesitsproteasomaldegradation AT jonmhuibregtse amaskedinitiationregioninretinoblastomaproteinregulatesitsproteasomaldegradation AT andreasmatouschek amaskedinitiationregioninretinoblastomaproteinregulatesitsproteasomaldegradation AT takuyatomita maskedinitiationregioninretinoblastomaproteinregulatesitsproteasomaldegradation AT jonmhuibregtse maskedinitiationregioninretinoblastomaproteinregulatesitsproteasomaldegradation AT andreasmatouschek maskedinitiationregioninretinoblastomaproteinregulatesitsproteasomaldegradation
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A masked initiation region in retinoblastoma protein regulates its proteasomal degradation

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