Real-Time PCR Assay for the Detection and Quantification of Roe Deer to Detect Food Adulteration—Interlaboratory Validation Involving Laboratories in Austria, Germany, and Switzerland

Game meat products are particularly prone to be adulterated by replacing game meat with cheaper meat species. Recently, we have presented a real-time polymerase chain reaction (PCR) assay for the identification and quantification of roe deer in food. Quantification of the roe deer content in % (<...

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Autores principales:	Barbara Druml, Steffen Uhlig, Kirsten Simon, Kirstin Frost, Karina Hettwer, Margit Cichna-Markl, Rupert Hochegger
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	real-time PCR roe deer game meat detection quantification food authentication Chemical technology TP1-1185
Acceso en línea:	https://doaj.org/article/f0174d8503f843d8a98eb560a213c33f
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spelling	oai:doaj.org-article:f0174d8503f843d8a98eb560a213c33f2021-11-25T17:33:47ZReal-Time PCR Assay for the Detection and Quantification of Roe Deer to Detect Food Adulteration—Interlaboratory Validation Involving Laboratories in Austria, Germany, and Switzerland10.3390/foods101126452304-8158https://doaj.org/article/f0174d8503f843d8a98eb560a213c33f2021-11-01T00:00:00Zhttps://www.mdpi.com/2304-8158/10/11/2645https://doaj.org/toc/2304-8158Game meat products are particularly prone to be adulterated by replacing game meat with cheaper meat species. Recently, we have presented a real-time polymerase chain reaction (PCR) assay for the identification and quantification of roe deer in food. Quantification of the roe deer content in % (<i>w</i>/<i>w</i>) was achieved relatively by subjecting the DNA isolates to a reference real-time PCR assay in addition to the real-time PCR assay for roe deer. Aiming at harmonizing analytical methods for food authentication across EU Member States, the real-time PCR assay for roe deer has been tested in an interlaboratory ring trial including 14 laboratories from Austria, Germany, and Switzerland. Participating laboratories obtained aliquots of DNA isolates from a meat mixture containing 24.8% (<i>w</i>/<i>w</i>) roe deer in pork, roe deer meat, and 12 meat samples whose roe deer content was not disclosed. Performance characteristics included amplification efficiency, level of detection (LOD<sub>95%</sub>), repeatability, reproducibility, and accuracy of quantitative results. With a relative reproducibility standard deviation ranging from 13.35 to 25.08% (after outlier removal) and recoveries ranging from 84.4 to 114.3%, the real-time PCR assay was found to be applicable for the detection and quantification of roe deer in raw meat samples to detect food adulteration.Barbara DrumlSteffen UhligKirsten SimonKirstin FrostKarina HettwerMargit Cichna-MarklRupert HocheggerMDPI AGarticlereal-time PCRroe deergame meatdetectionquantificationfood authenticationChemical technologyTP1-1185ENFoods, Vol 10, Iss 2645, p 2645 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	real-time PCR roe deer game meat detection quantification food authentication Chemical technology TP1-1185
spellingShingle	real-time PCR roe deer game meat detection quantification food authentication Chemical technology TP1-1185 Barbara Druml Steffen Uhlig Kirsten Simon Kirstin Frost Karina Hettwer Margit Cichna-Markl Rupert Hochegger Real-Time PCR Assay for the Detection and Quantification of Roe Deer to Detect Food Adulteration—Interlaboratory Validation Involving Laboratories in Austria, Germany, and Switzerland
description	Game meat products are particularly prone to be adulterated by replacing game meat with cheaper meat species. Recently, we have presented a real-time polymerase chain reaction (PCR) assay for the identification and quantification of roe deer in food. Quantification of the roe deer content in % (<i>w</i>/<i>w</i>) was achieved relatively by subjecting the DNA isolates to a reference real-time PCR assay in addition to the real-time PCR assay for roe deer. Aiming at harmonizing analytical methods for food authentication across EU Member States, the real-time PCR assay for roe deer has been tested in an interlaboratory ring trial including 14 laboratories from Austria, Germany, and Switzerland. Participating laboratories obtained aliquots of DNA isolates from a meat mixture containing 24.8% (<i>w</i>/<i>w</i>) roe deer in pork, roe deer meat, and 12 meat samples whose roe deer content was not disclosed. Performance characteristics included amplification efficiency, level of detection (LOD<sub>95%</sub>), repeatability, reproducibility, and accuracy of quantitative results. With a relative reproducibility standard deviation ranging from 13.35 to 25.08% (after outlier removal) and recoveries ranging from 84.4 to 114.3%, the real-time PCR assay was found to be applicable for the detection and quantification of roe deer in raw meat samples to detect food adulteration.
format	article
author	Barbara Druml Steffen Uhlig Kirsten Simon Kirstin Frost Karina Hettwer Margit Cichna-Markl Rupert Hochegger
author_facet	Barbara Druml Steffen Uhlig Kirsten Simon Kirstin Frost Karina Hettwer Margit Cichna-Markl Rupert Hochegger
author_sort	Barbara Druml
title	Real-Time PCR Assay for the Detection and Quantification of Roe Deer to Detect Food Adulteration—Interlaboratory Validation Involving Laboratories in Austria, Germany, and Switzerland
title_short	Real-Time PCR Assay for the Detection and Quantification of Roe Deer to Detect Food Adulteration—Interlaboratory Validation Involving Laboratories in Austria, Germany, and Switzerland
title_full	Real-Time PCR Assay for the Detection and Quantification of Roe Deer to Detect Food Adulteration—Interlaboratory Validation Involving Laboratories in Austria, Germany, and Switzerland
title_fullStr	Real-Time PCR Assay for the Detection and Quantification of Roe Deer to Detect Food Adulteration—Interlaboratory Validation Involving Laboratories in Austria, Germany, and Switzerland
title_full_unstemmed	Real-Time PCR Assay for the Detection and Quantification of Roe Deer to Detect Food Adulteration—Interlaboratory Validation Involving Laboratories in Austria, Germany, and Switzerland
title_sort	real-time pcr assay for the detection and quantification of roe deer to detect food adulteration—interlaboratory validation involving laboratories in austria, germany, and switzerland
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/f0174d8503f843d8a98eb560a213c33f
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Real-Time PCR Assay for the Detection and Quantification of Roe Deer to Detect Food Adulteration—Interlaboratory Validation Involving Laboratories in Austria, Germany, and Switzerland

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