Semisynthetic fluorescent pH sensors for imaging exocytosis and endocytosis

Existing pH-sensitive red fluorescent protein probes don’t perform well in monitoring exocytosis and endocytosis. Here, the authors combine organic dyes with self-labeling tags or antibodies to develop semisynthetic protein conjugates that can image synaptic vesicle fusion events in living cells.

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Auteurs principaux: Magalie Martineau, Agila Somasundaram, Jonathan B. Grimm, Todd D. Gruber, Daniel Choquet, Justin W. Taraska, Luke D. Lavis, David Perrais
Format: article
Langue:EN
Publié: Nature Portfolio 2017
Sujets:
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Accès en ligne:https://doaj.org/article/f0a9168f70654c7296d375712c1a1917
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