Semisynthetic fluorescent pH sensors for imaging exocytosis and endocytosis

Existing pH-sensitive red fluorescent protein probes don’t perform well in monitoring exocytosis and endocytosis. Here, the authors combine organic dyes with self-labeling tags or antibodies to develop semisynthetic protein conjugates that can image synaptic vesicle fusion events in living cells.

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Detalles Bibliográficos
Autores principales: Magalie Martineau, Agila Somasundaram, Jonathan B. Grimm, Todd D. Gruber, Daniel Choquet, Justin W. Taraska, Luke D. Lavis, David Perrais
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2017
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Acceso en línea:https://doaj.org/article/f0a9168f70654c7296d375712c1a1917
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Descripción
Sumario:Existing pH-sensitive red fluorescent protein probes don’t perform well in monitoring exocytosis and endocytosis. Here, the authors combine organic dyes with self-labeling tags or antibodies to develop semisynthetic protein conjugates that can image synaptic vesicle fusion events in living cells.