Semisynthetic fluorescent pH sensors for imaging exocytosis and endocytosis

Existing pH-sensitive red fluorescent protein probes don’t perform well in monitoring exocytosis and endocytosis. Here, the authors combine organic dyes with self-labeling tags or antibodies to develop semisynthetic protein conjugates that can image synaptic vesicle fusion events in living cells.

Guardado en:

Detalles Bibliográficos
Autores principales:	Magalie Martineau, Agila Somasundaram, Jonathan B. Grimm, Todd D. Gruber, Daniel Choquet, Justin W. Taraska, Luke D. Lavis, David Perrais
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/f0a9168f70654c7296d375712c1a1917
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:f0a9168f70654c7296d375712c1a1917
record_format	dspace
spelling	oai:doaj.org-article:f0a9168f70654c7296d375712c1a19172021-12-02T17:06:15ZSemisynthetic fluorescent pH sensors for imaging exocytosis and endocytosis10.1038/s41467-017-01752-52041-1723https://doaj.org/article/f0a9168f70654c7296d375712c1a19172017-11-01T00:00:00Zhttps://doi.org/10.1038/s41467-017-01752-5https://doaj.org/toc/2041-1723Existing pH-sensitive red fluorescent protein probes don’t perform well in monitoring exocytosis and endocytosis. Here, the authors combine organic dyes with self-labeling tags or antibodies to develop semisynthetic protein conjugates that can image synaptic vesicle fusion events in living cells.Magalie MartineauAgila SomasundaramJonathan B. GrimmTodd D. GruberDaniel ChoquetJustin W. TaraskaLuke D. LavisDavid PerraisNature PortfolioarticleScienceQENNature Communications, Vol 8, Iss 1, Pp 1-10 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Magalie Martineau Agila Somasundaram Jonathan B. Grimm Todd D. Gruber Daniel Choquet Justin W. Taraska Luke D. Lavis David Perrais Semisynthetic fluorescent pH sensors for imaging exocytosis and endocytosis
description	Existing pH-sensitive red fluorescent protein probes don’t perform well in monitoring exocytosis and endocytosis. Here, the authors combine organic dyes with self-labeling tags or antibodies to develop semisynthetic protein conjugates that can image synaptic vesicle fusion events in living cells.
format	article
author	Magalie Martineau Agila Somasundaram Jonathan B. Grimm Todd D. Gruber Daniel Choquet Justin W. Taraska Luke D. Lavis David Perrais
author_facet	Magalie Martineau Agila Somasundaram Jonathan B. Grimm Todd D. Gruber Daniel Choquet Justin W. Taraska Luke D. Lavis David Perrais
author_sort	Magalie Martineau
title	Semisynthetic fluorescent pH sensors for imaging exocytosis and endocytosis
title_short	Semisynthetic fluorescent pH sensors for imaging exocytosis and endocytosis
title_full	Semisynthetic fluorescent pH sensors for imaging exocytosis and endocytosis
title_fullStr	Semisynthetic fluorescent pH sensors for imaging exocytosis and endocytosis
title_full_unstemmed	Semisynthetic fluorescent pH sensors for imaging exocytosis and endocytosis
title_sort	semisynthetic fluorescent ph sensors for imaging exocytosis and endocytosis
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/f0a9168f70654c7296d375712c1a1917
work_keys_str_mv	AT magaliemartineau semisyntheticfluorescentphsensorsforimagingexocytosisandendocytosis AT agilasomasundaram semisyntheticfluorescentphsensorsforimagingexocytosisandendocytosis AT jonathanbgrimm semisyntheticfluorescentphsensorsforimagingexocytosisandendocytosis AT todddgruber semisyntheticfluorescentphsensorsforimagingexocytosisandendocytosis AT danielchoquet semisyntheticfluorescentphsensorsforimagingexocytosisandendocytosis AT justinwtaraska semisyntheticfluorescentphsensorsforimagingexocytosisandendocytosis AT lukedlavis semisyntheticfluorescentphsensorsforimagingexocytosisandendocytosis AT davidperrais semisyntheticfluorescentphsensorsforimagingexocytosisandendocytosis
_version_	1718381708620857344

Semisynthetic fluorescent pH sensors for imaging exocytosis and endocytosis

Ejemplares similares